Cloned (Comment) | Organism |
---|---|
functional expression both in Escherichia coli and HEK 293T cell | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lysophosphatidic acid | competitive | Rattus norvegicus | |
additional information | not inhibitory at 5 mM: Ca2+, Mg2+, Mn2+, EDTA, NaF or PMSF, phosphatidic acid, sphingosine 1-phosphate, monoacylglycerophosphocholine, and monoacylglycerophosphoethanolamine | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
lysoplasmenylcholine | pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized | Rattus norvegicus | |
0.053 | - |
lysoplasmenylethanolamine | pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | highest expression | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.76 | - |
pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysoplasmenylcholine + H2O | - |
Rattus norvegicus | an aldehyde + sn-glycero-3-phosphocholine | - |
? | |
lysoplasmenylethanolamine + H2O | - |
Rattus norvegicus | aldehyde + sn-glycero-3-phosphoethanolamine | - |
? | |
additional information | no substrate: 1-alkenyl-2-acyl-sn-glycero-3-phosphocholine or 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphocholine or 1-acyl-sn-glycero-3-phosphoethanolamine, and lysophosphatidic acid | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE | Rattus norvegicus |
More | enzyme is identical to protein Tmem86b, a hydrophobic transmembrane protein of the YhhN family | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
Tmem86b | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
lysoplasmenylcholine | pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized | Rattus norvegicus | |
10.3 | - |
lysoplasmenylethanolamine | pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | at least 50% of maximum activity within | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression in HEK 293T cells leads to decreased levels of plasmalogens | Rattus norvegicus |