Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.3.2.13 extracted from

  • Hubrich, F.; Mueller, M.; Andexer, J.N.
    In vitro production and purification of isochorismate using a two-enzyme cascade (2014), J. Biotechnol., 191, 93-98.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate + H2O Streptomyces hygroscopicus strict preference for chorismate 3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
subsp. ascomyceticus
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + H2O strict preference for chorismate Streptomyces hygroscopicus 3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
?
additional information isochorismate is not accepted as a substrate Streptomyces hygroscopicus ?
-
?

Synonyms

Synonyms Comment Organism
fkbO
-
Streptomyces hygroscopicus