Literature summary for 3.3.2.13 extracted from

Andexer, J.N.; Kendrew, S.G.; Nur-e-Alam, M.; Lazos, O.; Foster, T.A.; Zimmermann, A.S.; Warneck, T.D.; Suthar, D.; Coates, N.J.; Koehn, F.E.; Skotnicki, J.S.; Carter, G.T.; Gregory, M.A.; Martin, C.J.; Moss, S.J.; Leadlay, P.F.; Wilkinson, B.
Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate (2011), Proc. Natl. Acad. Sci. USA, 108, 4776-4781.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces hygroscopicus subsp. ascomyceticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	chorismate	in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C	Streptomyces hygroscopicus subsp. ascomyceticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chorismate + H2O	Streptomyces hygroscopicus subsp. ascomyceticus	-	(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces hygroscopicus subsp. ascomyceticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Streptomyces hygroscopicus subsp. ascomyceticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chorismate + H2O	-	Streptomyces hygroscopicus subsp. ascomyceticus	(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate	-	?

Synonyms

Synonyms	Comment	Organism
fkbO	-	Streptomyces hygroscopicus subsp. ascomyceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.6	-	chorismate	in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C	Streptomyces hygroscopicus subsp. ascomyceticus

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Release 2023.1 (January 2023)