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Literature summary for 3.3.2.13 extracted from

  • Juneja, P.; Hubrich, F.; Diederichs, K.; Welte, W.; Andexer, J.N.
    Mechanistic implications for the chorismatase FkbO based on the crystal structure (2014), J. Mol. Biol., 426, 105-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells Streptomyces hygroscopicus subsp. ascomyceticus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000 Streptomyces hygroscopicus subsp. ascomyceticus

Protein Variants

Protein Variants Comment Organism
E338A inactive Streptomyces hygroscopicus subsp. ascomyceticus
E338Q inactive Streptomyces hygroscopicus subsp. ascomyceticus
F226A the mutant shows 2.5% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus
R228A the mutant shows 224.5% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus
Y215F the mutant shows 12% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus

Inhibitors

Inhibitors Comment Organism Structure
3-(2-carboxyethyl)benzoate competitive inhibitor Streptomyces hygroscopicus subsp. ascomyceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
chorismate wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C Streptomyces hygroscopicus subsp. ascomyceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate + H2O Streptomyces hygroscopicus subsp. ascomyceticus
-
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus subsp. ascomyceticus Q9KID9
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 75 gel filtration Streptomyces hygroscopicus subsp. ascomyceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + H2O
-
Streptomyces hygroscopicus subsp. ascomyceticus (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
?

Synonyms

Synonyms Comment Organism
fkbO
-
Streptomyces hygroscopicus subsp. ascomyceticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.02
-
3-(2-carboxyethyl)benzoate wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C Streptomyces hygroscopicus subsp. ascomyceticus