Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells | Streptomyces hygroscopicus subsp. ascomyceticus |
Crystallization (Comment) | Organism |
---|---|
in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000 | Streptomyces hygroscopicus subsp. ascomyceticus |
Protein Variants | Comment | Organism |
---|---|---|
E338A | inactive | Streptomyces hygroscopicus subsp. ascomyceticus |
E338Q | inactive | Streptomyces hygroscopicus subsp. ascomyceticus |
F226A | the mutant shows 2.5% activity compared to the wild type enzyme | Streptomyces hygroscopicus subsp. ascomyceticus |
R228A | the mutant shows 224.5% activity compared to the wild type enzyme | Streptomyces hygroscopicus subsp. ascomyceticus |
Y215F | the mutant shows 12% activity compared to the wild type enzyme | Streptomyces hygroscopicus subsp. ascomyceticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(2-carboxyethyl)benzoate | competitive inhibitor | Streptomyces hygroscopicus subsp. ascomyceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
chorismate | wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C | Streptomyces hygroscopicus subsp. ascomyceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + H2O | Streptomyces hygroscopicus subsp. ascomyceticus | - |
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus subsp. ascomyceticus | Q9KID9 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 75 gel filtration | Streptomyces hygroscopicus subsp. ascomyceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + H2O | - |
Streptomyces hygroscopicus subsp. ascomyceticus | (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fkbO | - |
Streptomyces hygroscopicus subsp. ascomyceticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
3-(2-carboxyethyl)benzoate | wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C | Streptomyces hygroscopicus subsp. ascomyceticus |