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Literature summary for 3.3.2.12 extracted from
- Studies on the mechanism of ring hydrolysis in phenylacetate degradation: A metabolic branching point (2011), J. Biol. Chem., 286, 11021-11034.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
oxepin-CoA | pH and temperature not specified in the publication | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-CoA + H2O | the enzyme shows 1% activity with crotonyl-CoA compared to oxepin-CoA | Escherichia coli | (R)-3-hydroxybutyryl-CoA | - |
? | |
| additional information | the bifunctional protein also use 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde and NADP+ as substrate yielding 3-oxo-5,6-dehydrosuberyl-CoA as main product. The PaaZ-ECH domain acts as (R)-specific hydratase and shows no activity with (S)-3-hydroxybutyryl-CoA | Escherichia coli | ? | - |
? | |
| oxepin-CoA + H2O | 100% activity | Escherichia coli | 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde | main product | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ECH-Aa | - |
Escherichia coli |
| oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (NADP+) | bifunctional fusion proteom | Escherichia coli |
| paaZ | - |
Escherichia coli |
| PaaZ-ECH | domain showing oxepin-CoA hydrolase activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 39 | - |
oxepin-CoA | pH and temperature not specified in the publication | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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