Literature summary for 3.3.2.12 extracted from

Ismail, W.; El-Said Mohamed, M.; Wanner, B.; Datsenko, K.; Eisenreich, W.; Rohdich, F.; Bacher, A.; Fuchs, G.
Functional genomics by NMR spectroscopy: Phenylacetate catabolism in Escherichia coli (2003), Eur. J. Biochem., 270, 3047-3054.

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Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli	P77455	-	-

Synonyms

Synonyms	Comment	Organism
paaZ	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	Eschericha coli K12 mutants with a deletion of the paaZ gene are unable to grow with phenylacetate as carbon source. The paaZ mutant converts phenylacetate into ortho-hydroxyphenylacetate	Escherichia coli
physiological function	mutants with a deletion of the paaF, paaG, paaH, paaJ or paaZ gene are unable to grow with phenylacetate as carbon source. The paaG and paaZ mutants convert phenylacetate into ortho-hydroxyphenylacetate. Results suggest a catabolic pathway via CoA thioesters. Phenylacetyl-CoA is attacked by a ring-oxygenase/reductase, PaaABCDE proteins, generating a hydroxylated and reduced derivative of phenylacetyl-CoA, which is not re-oxidized to a dihydroxylated aromatic intermediate but is further metabolized in a complex reaction sequence comprising enoyl-CoA isomerization/hydration, nonoxygenolytic ring opening, and dehydrogenation catalyzed by the PaaG and PaaZ proteins. The subsequent beta-oxidation-type degradation of the resulting CoA dicarboxylate via beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA appears to be catalyzed by the PaaJ, PaaF and PaaH proteins	Escherichia coli

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Release 2023.1 (January 2023)