Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | activates at 3% v/v | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6alpha-imino-5alpha-cholestan-3beta-ol | strong, specific, competitive inhibition | Rattus norvegicus | |
7-dehydrocholesterol 5,6 beta-oxide | specific, mechanism-based inhibitor | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Long Evans rats | - |
Purification (Comment) | Organism |
---|---|
partially by microsome preparation | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol | positively charged transition state | Rattus norvegicus | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol | positively charged transition state | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol oxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics at pH 8.0, 37°C, 6.5% v/v acetonitrile | Rattus norvegicus |