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Literature summary for 3.3.2.11 extracted from
- Microsomal cholesterol epoxide hydrolase activity in 2-acetylaminofluorene-induced rat liver hyperplastic nodules (1987), Biochem. Pharmacol., 36, 2424-2427.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | maximal enzyme activity at 6.25% v/v | Rattus norvegicus |  |
| additional information | 2-acetylaminofluorene induces the enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 9-fluorenone | slight inhibition | Rattus norvegicus | |
| chalcone | - |
Rattus norvegicus | |
| ellipticine | - |
Rattus norvegicus | |
| harman | - |
Rattus norvegicus | |
| Isoquinoline | slight inhibition | Rattus norvegicus | |
| Metyrapone | weak inhibition in hyperplastic nodules, not in normal tissue | Rattus norvegicus | |
| additional information | comparison of inhibition level in microsomes of normal liver tissue and liver hyperplastic nodules, remaining activities, overview | Rattus norvegicus | |
| norharman | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00091 | - |
5,6-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, normal liver tissue microsomes | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | normal tissue and hyperplastic nodules | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00007 | - |
microsomes from hyperplastic nodules | Rattus norvegicus |
| 0.00017 | - |
microsomes from normal liver tissue | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cholesterol epoxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
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