Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
(S)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei | |
21.8 | - |
(R)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38200 | - |
calculated from sequence | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | G0R7E2 | - |
- |
Trichoderma reesei QM9414 | G0R7E2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-styrene oxide + H2O | low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide | Trichoderma reesei | (R)-styrene glycol | - |
? | |
(R)-styrene oxide + H2O | low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide | Trichoderma reesei QM9414 | (R)-styrene glycol | - |
? | |
(S)-styrene oxide + H2O | the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide | Trichoderma reesei | (S)-styrene glycol | - |
? | |
(S)-styrene oxide + H2O | the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide | Trichoderma reesei QM9414 | (S)-styrene glycol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 50 | highly active at temperatures ranging from 23°C to 50°C | Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme conserves 80% of its activity for at least 20 min | Trichoderma reesei |
50 | - |
activity decreases by 40% in the first 10 min, and 60% of the activity is preserved until 20 min of incubation | Trichoderma reesei |
70 | - |
t1/2: 7.4 min | Trichoderma reesei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
265 | - |
(S)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei | |
336 | - |
(R)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.2 | pH 6.0: about 55% of maximal activity, pH 8.2: about 40% of maximal activity | Trichoderma reesei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.6 | - |
(R)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei | |
88.3 | - |
(S)-styrene oxide | pH 7.0, 37°C | Trichoderma reesei |