Protein Variants | Comment | Organism |
---|---|---|
C154Y | the mutant has a significantly higher phosphatase activity than wild type | Homo sapiens |
K55R | the mutant has a significantly higher phosphatase activity than wild type | Homo sapiens |
R103C | the mutant has a significantly lower phosphatase activity than wild type | Homo sapiens |
R287Q | the mutation affects enzyme activity through reducing formation of a catalytically active dimer. The mutant has a significantly lower phosphatase activity than wild type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
trans-1,3-diphenylpropene oxide | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.004 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.0058 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
0.0059 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.006 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.007 | - |
14,15-epoxyeicosatrienoic acid | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.007 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.0074 | - |
AttoPhos | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.0074 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
0.0074 | - |
AttoPhos | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
0.009 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.0092 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.0097 | - |
AttoPhos | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.01 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.0101 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.011 | - |
1-myristoyl-sn-glycerol 3-phosphate | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.012 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.014 | - |
AttoPhos | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.017 | - |
AttoPhos | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.019 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34913 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-myristoyl-sn-glycerol 3-phosphate + H2O | - |
Homo sapiens | ? | - |
? | |
14,15-epoxyeicosatrienoic acid + H2O | - |
Homo sapiens | 14,15-dihydroxyeicosatrienoic acid | - |
? | |
AttoPhos + H2O | - |
Homo sapiens | ? | - |
? | |
trans-1,3-diphenylpropene oxide + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SEH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
AttoPhos | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.0046 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.005 | - |
AttoPhos | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.0131 | - |
AttoPhos | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.0182 | - |
AttoPhos | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
0.027 | - |
AttoPhos | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.068 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.076 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.15 | - |
1-myristoyl-sn-glycerol 3-phosphate | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
0.32 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
0.41 | - |
1-myristoyl-sn-glycerol 3-phosphate | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
0.44 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.76 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.99 | - |
trans-1,3-diphenylpropene oxide | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
1.72 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
2.1 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
2.55 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
5 | - |
14,15-epoxyeicosatrienoic acid | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
7.5 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
15 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
AttoPhos | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
0.36 | - |
AttoPhos | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
0.4 | - |
1-myristoyl-glycerol-3-phosphate | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
1.35 | - |
AttoPhos | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
2.5 | - |
AttoPhos | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
3.63 | - |
AttoPhos | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
8 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
10 | - |
1-myristoyl-glycerol-3-phosphate | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
14 | - |
1-myristoyl-glycerol-3-phosphate | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
22 | - |
1-myristoyl-glycerol-3-phosphate | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
50 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R287Q, at pH 7.4 and 30°C | Homo sapiens | |
52 | - |
1-myristoyl-glycerol-3-phosphate | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
130 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
210 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme R103C, at pH 7.4 and 30°C | Homo sapiens | |
300 | - |
trans-1,3-diphenylpropene oxide | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
300 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
640 | - |
trans-1,3-diphenylpropene oxide | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens | |
710 | - |
14,15-epoxyeicosatrienoic acid | wild type enzyme, at pH 7.4 and 30°C | Homo sapiens | |
1000 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme K55R, at pH 7.4 and 30°C | Homo sapiens | |
1600 | - |
14,15-epoxyeicosatrienoic acid | mutant enzyme C154Y, at pH 7.4 and 30°C | Homo sapiens |