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Literature summary for 3.3.2.10 extracted from

  • Morisseau, C.; Wecksler, A.T.; Deng, C.; Dong, H.; Yang, J.; Lee, K.S.; Kodani, S.D.; Hammock, B.D.
    Effect of soluble epoxide hydrolase polymorphism on substrate and inhibitor selectivity and dimer formation (2014), J. Lipid Res., 55, 1131-1138.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.10

Protein Variants

Protein Variants Comment Organism
C154Y the mutant has a significantly higher phosphatase activity than wild type Homo sapiens
K55R the mutant has a significantly higher phosphatase activity than wild type Homo sapiens
R103C the mutant has a significantly lower phosphatase activity than wild type Homo sapiens
R287Q the mutation affects enzyme activity through reducing formation of a catalytically active dimer. The mutant has a significantly lower phosphatase activity than wild type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 trans-1,3-diphenylpropene oxide wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.004
-
 trans-1,3-diphenylpropene oxide mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.0058
-
 trans-1,3-diphenylpropene oxide mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
0.0059
-
 trans-1,3-diphenylpropene oxide mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.006
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.007
-
 14,15-epoxyeicosatrienoic acid wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.007
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.0074
-
 AttoPhos mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.0074
-
 14,15-epoxyeicosatrienoic acid mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
0.0074
-
 AttoPhos mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
0.009
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.0092
-
 14,15-epoxyeicosatrienoic acid mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.0097
-
 AttoPhos wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.01
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.0101
-
 trans-1,3-diphenylpropene oxide mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.011
-
 1-myristoyl-sn-glycerol 3-phosphate wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.012
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.014
-
 AttoPhos mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.017
-
 AttoPhos mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.019
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-myristoyl-sn-glycerol 3-phosphate + H2O
-
 Homo sapiens ?
-
 ?
14,15-epoxyeicosatrienoic acid + H2O
-
 Homo sapiens 14,15-dihydroxyeicosatrienoic acid
-
 ?
AttoPhos + H2O
-
 Homo sapiens ?
-
 ?
trans-1,3-diphenylpropene oxide + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
SEH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 AttoPhos mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.0046
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.005
-
 AttoPhos mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.0131
-
 AttoPhos wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.0182
-
 AttoPhos mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
0.027
-
 AttoPhos mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.068
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.076
-
 trans-1,3-diphenylpropene oxide mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.15
-
 1-myristoyl-sn-glycerol 3-phosphate wild type enzyme, at pH 7.4 and 30°C Homo sapiens
0.32
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
0.41
-
 1-myristoyl-sn-glycerol 3-phosphate mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
0.44
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.76
-
 trans-1,3-diphenylpropene oxide mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.99
-
 trans-1,3-diphenylpropene oxide wild type enzyme, at pH 7.4 and 30°C Homo sapiens
1.72
-
 trans-1,3-diphenylpropene oxide mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
2.1
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
2.55
-
 trans-1,3-diphenylpropene oxide mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
5
-
 14,15-epoxyeicosatrienoic acid wild type enzyme, at pH 7.4 and 30°C Homo sapiens
7.5
-
 14,15-epoxyeicosatrienoic acid mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
15
-
 14,15-epoxyeicosatrienoic acid mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
 AttoPhos mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
0.36
-
 AttoPhos mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
0.4
-
 1-myristoyl-glycerol-3-phosphate mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
1.35
-
 AttoPhos wild type enzyme, at pH 7.4 and 30°C Homo sapiens
2.5
-
 AttoPhos mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
3.63
-
 AttoPhos mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
8
-
 trans-1,3-diphenylpropene oxide mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
10
-
 1-myristoyl-glycerol-3-phosphate mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
14
-
 1-myristoyl-glycerol-3-phosphate wild type enzyme, at pH 7.4 and 30°C Homo sapiens
22
-
 1-myristoyl-glycerol-3-phosphate mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
50
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R287Q, at pH 7.4 and 30°C Homo sapiens
52
-
 1-myristoyl-glycerol-3-phosphate mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
130
-
 trans-1,3-diphenylpropene oxide mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
210
-
 14,15-epoxyeicosatrienoic acid mutant enzyme R103C, at pH 7.4 and 30°C Homo sapiens
300
-
 trans-1,3-diphenylpropene oxide wild type enzyme, at pH 7.4 and 30°C Homo sapiens
300
-
 trans-1,3-diphenylpropene oxide mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
640
-
 trans-1,3-diphenylpropene oxide mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens
710
-
 14,15-epoxyeicosatrienoic acid wild type enzyme, at pH 7.4 and 30°C Homo sapiens
1000
-
 14,15-epoxyeicosatrienoic acid mutant enzyme K55R, at pH 7.4 and 30°C Homo sapiens
1600
-
 14,15-epoxyeicosatrienoic acid mutant enzyme C154Y, at pH 7.4 and 30°C Homo sapiens