Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
E88R/T102A/M368I/K398E/G412D | the variant possesses (S)-styrene oxide-preferred hydrolytic activity | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.47 | - |
(R)-styrene oxide | wild type enzyme, at pH 6.0 and 10°C | Danio rerio | |
7.85 | - |
(S)-styrene oxide | wild type enzyme, at pH 6.0 and 10°C | Danio rerio | |
7.95 | - |
(R)-styrene oxide | mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C | Danio rerio | |
8.33 | - |
(S)-styrene oxide | mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Danio rerio | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64800 | - |
x * 64800, SDS-PAGE | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-styrene oxide + H2O | Danio rerio | - |
(R)-styrene glycol | - |
? | |
(S)-styrene oxide + H2O | Danio rerio | the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity | (S)-styrene glycol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q5PRC6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-styrene oxide + H2O | - |
Danio rerio | (R)-styrene glycol | - |
? | |
(S)-styrene oxide + H2O | the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity | Danio rerio | (S)-styrene glycol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64800, SDS-PAGE | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
EPHX2 | - |
Danio rerio |
SEH | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Danio rerio |