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Literature summary for 3.3.2.10 extracted from
- Isolation and characterization of Xenopus soluble epoxide hydrolase (2014), Biochim. Biophys. Acta, 1841, 954-962.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus (DE3) cells | Xenopus laevis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N29T/H146R | the mutant shows strongly reduced hydrolase activity and does not have phosphatase activity | Xenopus laevis |
| N29T/H146R/G11D | the mutant shows strongly reduced hydrolase activity and does not have phosphatase activity | Xenopus laevis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01916 | - |
3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester 2-oxiraneacetic acid | at pH 7.0 and 30°C | Xenopus laevis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Xenopus laevis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xenopus laevis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Xenopus laevis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | lowest expression | Xenopus laevis | - |
| embryo | - |
Xenopus laevis | - |
| eye | - |
Xenopus laevis | - |
| heart | - |
Xenopus laevis | - |
| kidney | - |
Xenopus laevis | - |
| liver | highest expression | Xenopus laevis | - |
| lung | - |
Xenopus laevis | - |
| pancreas | - |
Xenopus laevis | - |
| spleen | - |
Xenopus laevis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11,12-epoxyeicosatrienoic acid + H2O | - |
Xenopus laevis | 11,12-dihydroxyeicosatrienoic acid | - |
? | |
| 14,15-epoxyeicosatrienoic acid + H2O | - |
Xenopus laevis | 14,15-dihydroxyeicosatrienoic acid | - |
? | |
| 3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester 2-oxiraneacetic acid + H2O | - |
Xenopus laevis | 6-methoxy-2-naphthaldehyde + ? | - |
? | |
| additional information | the enzyme does not show phosphatase activity toward 4-methylumbelliferyl phosphate or several lysophosphatidic acids | Xenopus laevis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SEH | - |
Xenopus laevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Xenopus laevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Xenopus laevis |
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Release 2023.1 (January 2023)
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