Cloned (Comment) | Organism |
---|---|
sEH cloning from liver, expression of N-terminal His-tagged sEH in Escherichia coli strain BL21(DE3) by auto-induction, subcloning in Escherichia coli strain DH5alpha, method development, overview. Expression of sEH in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, Method comparisons, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | - |
Homo sapiens | |
1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | - |
Homo sapiens | |
1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | - |
Homo sapiens | |
1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea | - |
Homo sapiens | |
12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid | - |
Homo sapiens | |
butyl 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoate | - |
Homo sapiens | |
additional information | Inhibitors of human sEH are effective drug candidates for the treatment of cardiovascular diseases, inhibitor screening with recombinant enzyme, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34913 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged sEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.315 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens |
4.044 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyano-(2-methoxynaphthalen-6-yl)-methyl trans-(3-phenyl-oxiran-2-yl)-methyl carbonate + H2O | CMNPC, a fluorescent substrate with an epoxide group, which releases a strong fluorophore 6-methoxy-2-naphthaldehyde after hydrolysis of the epoxide | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SEH | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000001 | - |
recombinant enzyme expressed in Escherichia coli or Sf9 cells | Homo sapiens | butyl 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoate | |
0.0000022 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens | 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid | |
0.0000023 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens | 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid | |
0.000008 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens | 1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000009 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens | 1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.00012 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens | 1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000128 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens | 1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000206 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens | 1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000258 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens | 1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000613 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens | 1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea | |
0.000813 | - |
recombinant enzyme expressed in Sf9 cells | Homo sapiens | 1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea |
General Information | Comment | Organism |
---|---|---|
physiological function | soluble epoxide hydrolase is a key enzyme involved in the metabolism of epoxy fatty acid mediators such as epoxyeicosatrienoic acids with emerging roles in the regulations of hypertension and inflammation | Homo sapiens |