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Literature summary for 3.3.2.10 extracted from

  • Nishi, K.; Kim, I.H.; Ma, S.J.
    Expression of the human soluble epoxide hydrolase in Escherichia coli by auto-induction for the study of high-throughput inhibition assays (2010), Protein Expr. Purif., 69, 34-38.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sEH cloning from liver, expression of N-terminal His-tagged sEH in Escherichia coli strain BL21(DE3) by auto-induction, subcloning in Escherichia coli strain DH5alpha, method development, overview. Expression of sEH in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, Method comparisons, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
-
Homo sapiens
1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
-
Homo sapiens
1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
-
Homo sapiens
1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea
-
Homo sapiens
12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid
-
Homo sapiens
butyl 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoate
-
Homo sapiens
additional information Inhibitors of human sEH are effective drug candidates for the treatment of cardiovascular diseases, inhibitor screening with recombinant enzyme, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged sEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.315
-
recombinant enzyme expressed in Escherichia coli Homo sapiens
4.044
-
recombinant enzyme expressed in Sf9 cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyano-(2-methoxynaphthalen-6-yl)-methyl trans-(3-phenyl-oxiran-2-yl)-methyl carbonate + H2O CMNPC, a fluorescent substrate with an epoxide group, which releases a strong fluorophore 6-methoxy-2-naphthaldehyde after hydrolysis of the epoxide Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
SEH
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000001
-
recombinant enzyme expressed in Escherichia coli or Sf9 cells Homo sapiens butyl 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoate
0.0000022
-
recombinant enzyme expressed in Escherichia coli Homo sapiens 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid
0.0000023
-
recombinant enzyme expressed in Sf9 cells Homo sapiens 12-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamoyl)amino]dodecanoic acid
0.000008
-
recombinant enzyme expressed in Escherichia coli Homo sapiens 1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000009
-
recombinant enzyme expressed in Sf9 cells Homo sapiens 1-[5-[2-(2-ethoxyethoxy)ethoxy]pentyl]-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.00012
-
recombinant enzyme expressed in Escherichia coli Homo sapiens 1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000128
-
recombinant enzyme expressed in Sf9 cells Homo sapiens 1-(5-hydroxypentyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000206
-
recombinant enzyme expressed in Escherichia coli Homo sapiens 1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000258
-
recombinant enzyme expressed in Sf9 cells Homo sapiens 1-(2-hydroxyphenyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000613
-
recombinant enzyme expressed in Escherichia coli Homo sapiens 1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea
0.000813
-
recombinant enzyme expressed in Sf9 cells Homo sapiens 1-(3-hydroxypropyl)-3-tricyclo[3.3.1.13,7]dec-1-ylurea

General Information

General Information Comment Organism
physiological function soluble epoxide hydrolase is a key enzyme involved in the metabolism of epoxy fatty acid mediators such as epoxyeicosatrienoic acids with emerging roles in the regulations of hypertension and inflammation Homo sapiens