Literature summary for 3.3.2.10 extracted from

Harris, T.R.; Morisseau, C.; Walzem, R.L.; Ma, S.J.; Hammock, B.D.
The cloning and characterization of a soluble epoxide hydrolase in chicken (2006), Poult. Sci., 85, 278-287.

Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Spodoptera frugiperda Sf21 cells or in Trichoplusia ni Tn5 cells using the baculovirus infection system	Gallus gallus

Inhibitors

Inhibitors	Comment	Organism	Structure
1-cyclohexyl-3-dodecylurea	i.e. 1-cyclohexyl-3-dodecyl-urea, strong inhibition	Gallus gallus
1-cyclohexyl-3-ethylurea	i.e. N-cyclohexyl-N'-ethylurea	Gallus gallus
AUDA	i.e. 12-(3-adamantan-1-yl-ureido) dodecanoic acid, strong inhibition	Gallus gallus
N,N-dicyclohexylurea	i.e. N,N-dicyclohexylurea	Gallus gallus
N-adamantyl-N'-cyclohexylurea	i.e. N-adamantyl-N'-cyclohexylurea, strong inhibition	Gallus gallus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0253	-	trans-1,3-diphenylpropene oxide	pH 7.4, 30°C, purified recombinant enzyme	Gallus gallus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Gallus gallus	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	x * 63000, recombinant enzyme, SDS-PAGE	Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	Q45QT1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Sf21 or Tn5 insect cells by nickel affinity chromatography	Gallus gallus

Reaction

Reaction	Comment	Organism	Reaction ID
an epoxide + H2O = a glycol	the catalytic triad is Asp334-His523-Asp495	Gallus gallus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Gallus gallus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.003	-	purified recombinant enzyme, substrate trans-9,10-epoxystearate	Gallus gallus
0.012	-	purified recombinant enzyme, substrate cis-8,9-epoxyeicosatrienoic acid or cis-11,12-epoxyeicosatrienoic acid	Gallus gallus
0.025	-	purified recombinant enzyme, substrate cis-14,15-epoxyeicosatrienoic acid	Gallus gallus
0.823	-	purified recombinant enzyme, substrate trans-1,3-diphenylpropene oxide	Gallus gallus

Storage Stability

Storage Stability	Organism
25°C, purified recombinant enzyme, half-life is 9-24 h	Gallus gallus
37°C, purified recombinant enzyme, half-life is below 3 h	Gallus gallus
4°C, purified recombinant enzyme, half-life is more than 6 days	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cis-11,12-epoxyeicosatrienoic acid + H2O	-	Gallus gallus	cis-11,12-dihydroxyeicosatrienoic acid	-	?
cis-14,15-epoxyeicosatrienoic acid + H2O	preferred eicosatrienoic acid-substrate	Gallus gallus	cis-14,15-dihydroxyeicosatrienoic acid	-	?
cis-8,9-epoxyeicosatrienoic acid + H2O	-	Gallus gallus	cis-8,9-dihydroxyeicosatrienoic acid	-	?
trans-1,3-diphenylpropene oxide + H2O	the His-tag of the recombinant enzyme probably interfers with the enzyme activity	Gallus gallus	?	-	?
trans-9,10-epoxystearate + H2O	-	Gallus gallus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 63000, recombinant enzyme, SDS-PAGE	Gallus gallus

Synonyms

Synonyms	Comment	Organism
SEH	-	Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.9	-	trans-1,3-diphenylpropene oxide	pH 7.4, 30°C, purified recombinant enzyme	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Gallus gallus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Gallus gallus	isoelectric focusing	-	6.1

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Release 2023.1 (January 2023)