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Literature summary for 3.3.2.10 extracted from

  • Stapleton, A.; Beetham, J.K.; Pinot, F.; Garbarino, J.E.; Rockhold, D.R.; Friedman, M.; Hammock, B.D.; Belknap, W.R.
    Cloning and expression of soluble epoxide hydrolase from potato (1994), Plant J., 6, 251-258.
    View publication on PubMed
Search for more literature for 3.3.2.10

Activating Compound

Activating Compound Comment Organism Structure
additional information wounding and methyl jasmonate induce enzyme expression in leaves, while abscisic acid does not Solanum tuberosum

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of the potato enzyme in Spodoptera frugiperda cells and in Trichoplusia ni cells using the baculovirus infection system Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
4-fluorochalcone oxide complete inhibition of recombinant and native enzyme at 0.057 mM Solanum tuberosum
trans-(2R,3R)-3-phenylglycidol 23% inhibition of recombinant and of native enzyme at 0.057 mM Solanum tuberosum
trans-(2S,3S)-3-phenylglycidol 98% inhibition of recombinant and 85% inhibition of native enzyme at 0.057 mM Solanum tuberosum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Solanum tuberosum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, recombinant enzyme from insect cells, SDS-PAGE Solanum tuberosum

Organism

Organism UniProt Comment Textmining
no activity in Trichoplusia ni
-
 cell culture
-
Solanum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf meristem, in expanding leaves, not in mature leaves Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0014
-
 crude potato enzyme extract Solanum tuberosum
0.063
-
 recombinant enzyme in crude enzyme extract Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-stilbene oxide + H2O
-
 Solanum tuberosum 1,2-diphenylethane-1,2-diol
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, recombinant enzyme from insect cells, SDS-PAGE Solanum tuberosum