Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | clofibrate induces the enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methylglycidyl 4-nitrobenzoate | IC50 for the S-enantiomer is 0.717 mM, the R-enantiomer shows 25% inhibition at 0.2 mM | Mus musculus | |
3,3-dimethylglycidyl 4-nitrobenzoate | IC50 for the S-enantiomer is 0.012 mM, 23% inhibition at 5 mM of the R-enantiomer | Mus musculus | |
glycidyl 4-nitrobenzoate | IC50 for the S-enantiomer is 0.14 mM, and for the R-enantiomer 0.51 mM | Mus musculus | |
additional information | sterical differences play a role in inhibitory potency | Mus musculus | |
trans-(2R,3R)-3-phenylglycidol | IC50 for the R-enantiomer is about 3.9 mM | Mus musculus | |
trans-(2S,3S)-3-phenylglycidol | IC50 for the S-enantiomer is about 2.2 mM | Mus musculus | |
trans-1-phenylpropylene oxide | IC50 for the S-enantiomer is 3.47 mM, and for the R-enantiomer 2.98 mM | Mus musculus | |
trans-2-methyl-3-phenylglycidol | IC50 for the S-enantiomer is 1.52 mM, and for the R-enantiomer 2.0 mM | Mus musculus | |
trans-3-(4-bromophenyl)glycidol | IC50 for the S-enantiomer is 0.12 mM, and for the R-enantiomer 0.77 mM | Mus musculus | |
trans-3-(4-nitrophenyl)glycidol | IC50 for the S-enantiomer is 0.013 mM, and for the R-enantiomer 4.24 mM | Mus musculus | |
trans-3-(4-nitrophenyl)glycidyl acetate | IC50 for the S-enantiomer is 0.232 mM, and for the R-enantiomer 0.39 mM | Mus musculus | |
trans-3-(4-nitrophenyl)glycidyl benzoate | IC50 for the S-enantiomer is 0.229 mM, and for the R-enantiomer 0.10 mM | Mus musculus | |
trans-3-methylglycidyl 4-nitrobenzoate | the S-enantiomer shows 20% inhibition at 0.8 mM, the R-enantiomer 16.5% inhibition at 0.8 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mus musculus | |
0.008 | - |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate | pH 7.4, 25°C | Mus musculus | |
0.015 | - |
4-nitrophenyl (2R,3R)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate | pH 7.4, 25°C | Mus musculus | |
0.033 | - |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-phenylpropyl carbonate | pH 7.4, 25°C | Mus musculus | |
0.068 | - |
4-nitrophenyl (2R,3R)-2,3-epoxy-3-phenylpropyl carbonate | pH 7.4, 25°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
Swiss Webster mice | - |
Purification (Comment) | Organism |
---|---|
from liver by affinity chromatography on an epoxy activated resin derivatized with benzyl thiol | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl (2R,3R)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate + H2O | - |
Mus musculus | ? | - |
? | |
4-nitrophenyl (2R,3R)-2,3-epoxy-3-phenylpropyl carbonate + H2O | - |
Mus musculus | ? | - |
? | |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate + H2O | preferred phenylpropyl carbonate substrate | Mus musculus | ? | - |
? | |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-phenylpropyl carbonate + H2O | - |
Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cytosolic epoxide hydrolase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
37 | - |
inhibition assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
4-nitrophenyl (2R,3R)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate | pH 7.4, 25°C | Mus musculus | |
7.7 | - |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)propyl carbonate | pH 7.4, 25°C | Mus musculus | |
15.8 | - |
4-nitrophenyl (2S,3S)-2,3-epoxy-3-phenylpropyl carbonate | pH 7.4, 25°C | Mus musculus | |
26.7 | - |
4-nitrophenyl (2R,3R)-2,3-epoxy-3-phenylpropyl carbonate | pH 7.4, 25°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.012 | - |
IC50 for the S-enantiomer is 0.012 mM, 23% inhibition at 5 mM of the R-enantiomer | Mus musculus | 3,3-dimethylglycidyl 4-nitrobenzoate | |
0.013 | - |
IC50 for the S-enantiomer is 0.013 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidol | |
0.1 | - |
IC50 for the R-enantiomer 0.10 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidyl benzoate | |
0.12 | - |
IC50 for the S-enantiomer is 0.12 mM | Mus musculus | trans-3-(4-bromophenyl)glycidol | |
0.14 | - |
IC50 for the S-enantiomer is 0.14 mM | Mus musculus | glycidyl 4-nitrobenzoate | |
0.229 | - |
IC50 for the S-enantiomer is 0.229 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidyl benzoate | |
0.232 | - |
IC50 for the S-enantiomer is 0.232 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidyl acetate | |
0.39 | - |
IC50 for the R-enantiomer 0.39 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidyl acetate | |
0.514 | - |
IC50 for the R-enantiomer 0.514 mM | Mus musculus | glycidyl 4-nitrobenzoate | |
0.717 | - |
IC50 for the S-enantiomer is 0.717 mM, the R-enantiomer shows 25% inhibition at 0.2 mM | Mus musculus | 2-methylglycidyl 4-nitrobenzoate | |
0.77 | - |
IC50 for the R-enantiomer 0.77 mM | Mus musculus | trans-3-(4-bromophenyl)glycidol | |
1.52 | - |
IC50 for the S-enantiomer is 1.52 mM | Mus musculus | trans-2-methyl-3-phenylglycidol | |
2 | - |
IC50 for the R-enantiomer 2.0 mM | Mus musculus | trans-2-methyl-3-phenylglycidol | |
2.2 | - |
IC50 for the S-enantiomer is about 2.2 mM | Mus musculus | trans-(2S,3S)-3-phenylglycidol | |
2.98 | - |
IC50 for the R-enantiomer 2.98 mM | Mus musculus | trans-1-phenylpropylene oxide | |
3.47 | - |
IC50 for the S-enantiomer is 3.47 mM | Mus musculus | trans-1-phenylpropylene oxide | |
3.9 | - |
IC50 for the R-enantiomer is about 3.9 mM | Mus musculus | trans-(2R,3R)-3-phenylglycidol | |
4.24 | - |
IC50 for the R-enantiomer 4.24 mM | Mus musculus | trans-3-(4-nitrophenyl)glycidol |