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Literature summary for 3.3.2.10 extracted from

  • Arand, M.; Hemmer, H.; Duerk, H.; Barattis, J.; Archelas, A.; Furstoss, R.
    Cloning and molecular characterization of a soluble epoxide hydrolase from Aspergillus niger that is related to mammalian microsomal epoxide hydrolase (1999), Biochem. J., 344, 273-280.
No PubMed abstract available
Search for more literature for 3.3.2.10

Application

Application Comment Organism
synthesis the enzyme is useful for enantioselective bio-organic synthesis of chiral substances Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, functional expression of wild-type enzyme and expression of enzyme mutants in Escherichia coli strain BL21(DE3) Aspergillus niger

Protein Variants

Protein Variants Comment Organism
D192A site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
D192N site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
D192S site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
D348A site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
D348E site directed mutagenesis, the mutant shows 48% of wild-type enzyme activity Aspergillus niger
H374K site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
H374N site directed mutagenesis, inactive or nearly inactive mutant Aspergillus niger
H374S site directed mutagenesis, inactive or nearly inactive mutant, recombinantly expressed mutant enzyme is not soluble but remains in the particulate fraction of Escherichia coli cells Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, 3.3.2.9 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 recombinant enzyme, gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q9UR30
-
-
Aspergillus niger LCP521 Q9UR30
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration Aspergillus niger

Reaction

Reaction Comment Organism Reaction ID
an epoxide + H2O = a glycol the catalytic triad is formed by Asp192, Asp348, and His374 Aspergillus niger
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-4-nitrostyrene oxide + H2O
-
 Aspergillus niger ?
-
 ?
(R)-4-nitrostyrene oxide + H2O
-
 Aspergillus niger LCP521 ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, EC 3.3.2.9 Aspergillus niger

Synonyms

Synonyms Comment Organism
EH
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Aspergillus niger