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Literature summary for 3.3.2.10 extracted from
- Polymorphisms in human soluble epoxide hydrolase: effects on enzyme activity, enzyme stability, and quarternary structure (2004), Arch. Biochem. Biophys., 427, 164-169.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EPHX2, expression of His-tagged wild-type and mutant enzymes in Spodoptera frugiperda Sf21 cells using the baculovirus infection system | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C154Y | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 2.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
| E470G | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
| K55R | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R103C | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.3, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R103C/R287Q | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
| R287Q | site-directed mutagenesis, the mutant shows the same hydrolase/phosphatase ratio of 1.0 as the wild-type, but reduced stability at 37°C | Homo sapiens |
| V422A | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.8, compared to the wild-type ratio of 1.0 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene EPHX2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Sf21 insect cells by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-stilbene oxide + H2O | - |
Homo sapiens | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the C-terminal part harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC 3.1.3.76 is located at the N-terminal part, Arg287 may be involved in formation of the quaternary enzyme structure | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | see also EC 3.1.3.76 | Homo sapiens |
| SEH | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
inactivation rate constants for recombinant wild-type and mutant enzymes | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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