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Literature summary for 3.3.2.10 extracted from

  • Li, C.; Liu, Q.; Song, X.; Ding, D.; Ji, A.; Qu, Y.
    Epoxide hydrolase-catalyzed resolution of ethyl 3-phenylglycidate using whole cells of Pseudomonas sp (2003), Biotechnol. Lett., 25, 2113-2116.
    View publication on PubMed
Search for more literature for 3.3.2.10

Activating Compound

Activating Compound Comment Organism Structure
additional information maximal activity with dimethyl formamide as co-solvent, effects of solvents on enzyme activity, overview Pseudomonas sp.

Application

Application Comment Organism
synthesis the enantioselective enzyme is useful in production of chiral substances, e.g. production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat by whole cells of Pseudomonas sp. strain BZS21, maximal activity with dimethyl formamide as co-solvent Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. BZS21
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:styrene oxide-grown cell
-
 Pseudomonas sp.
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culture condition:styrene-grown cell
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
racemic ethyl 3-phenylglycidate + H2O production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat Pseudomonas sp. ?
-
 ?
racemic ethyl 3-phenylglycidate + H2O production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat Pseudomonas sp. BZS21 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas sp.