Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D333G | no activity | Rattus norvegicus |
H332Q | strong decrease in enzymatic activity due to a change in substrate affinity, Km deviates only slightly from that of the wild-type enzyme, Vmax is reduced to 5% of the wild-type enzyme | Rattus norvegicus |
H517Y/C521Y | no activity | Rattus norvegicus |
H523D | no activity | Rattus norvegicus |
W334F | 7fold increase in Km-value, 2fold enhanced Vmax | Rattus norvegicus |
W540L | no activity | Rattus norvegicus |
W540L | strong decrease in enzymatic activity due to a change in substrate affinity, Km deviates only slightly from that of the wild-type enzyme | Rattus norvegicus |
W540S | no activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-fluorochalcone oxide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
trans-stilbene oxide | mutant enzyme W540L | Rattus norvegicus | |
0.007 | - |
trans-stilbene oxide | native enzyme | Rattus norvegicus | |
0.008 | - |
trans-stilbene oxide | recombinant fusion protein expressed in E. coli | Rattus norvegicus | |
0.0085 | - |
trans-stilbene oxide | mutant enzyme H332Q | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Asp333, Asp495 and His523 form the catalytic triad | Rattus norvegicus | ? | - |
? | |
trans-stilbene oxide + H2O | - |
Rattus norvegicus | ? | - |
? |