Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vibrio cholerae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 50 mM CaCl2, 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 550, 1% (w/v) n-octyl-beta-D-glucoside at 20°C | Vibrio cholerae |
Protein Variants | Comment | Organism |
---|---|---|
D35N | the mutation results in almost complete loss of enzyme activity | Vibrio cholerae |
H34A | the mutant has about 80% of wild type activity | Vibrio cholerae |
H34N | the mutant has about 35% of wild type activity | Vibrio cholerae |
K118A | the mutant has about 30% of wild type activity | Vibrio cholerae |
K118H | the mutation results in almost complete loss of enzyme activity | Vibrio cholerae |
K118N | the mutation results in almost complete loss of enzyme activity | Vibrio cholerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate + H2O | Vibrio cholerae | - |
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
isochorismate + H2O | Vibrio cholerae N16961 | - |
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | P0C6D3 | O1 biovar El Tor | - |
Vibrio cholerae N16961 | P0C6D3 | O1 biovar El Tor | - |
Purification (Comment) | Organism |
---|---|
Ni2+ chelating Sepharose column chromatography and Superdex 200 gel filtration | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate + H2O | - |
Vibrio cholerae | (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
isochorismate + H2O | - |
Vibrio cholerae N16961 | (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ISC | - |
Vibrio cholerae |