Cloned (Comment) | Organism |
---|---|
into the pGEM7z and the pET15bTEV vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of the EntB protein is determined at a resolution of 2.3 A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D227R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
D240R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
D244R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
D263R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
F264E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
I239D | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
K269E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
M249E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
R247E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
S245A | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33090 | - |
calculated molecular mass | Escherichia coli |
33100 | - |
determinecd by MALDI-TOF spectrometry | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ADI4 | - |
- |
Purification (Comment) | Organism |
---|---|
purification is obtained by two metal chelate chromatography steps seperated by emoval of the His tag with TEV protease | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EntB | contains an N-terminal isochorismate lyase domain | Escherichia coli |