Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Escherichia coli | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Escherichia coli |
enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
ethylthio-immucillin-A | - |
Escherichia coli | |
ethylthio-immucillin-A | - |
Streptococcus pneumoniae | |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
methylthio-immucillin-A | weak | Escherichia coli | |
methylthio-immucillin-A | weak | Streptococcus pneumoniae | |
p-tolylthio-immucillin-A | - |
Escherichia coli | |
p-tolylthio-immucillin-A | - |
Streptococcus pneumoniae | |
phenylthio-immucillin-A | - |
Escherichia coli | |
phenylthio-immucillin-A | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.023 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.43 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli | |
1.3 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Streptococcus pneumoniae | - |
bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Streptococcus pneumoniae | 5-methylthio-D-ribose + adenine | - |
? | |
additional information | no substrate: adenosine, inosine | Escherichia coli | ? | - |
? | |
additional information | no substrate: adenosine, inosine | Streptococcus pneumoniae | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Streptococcus pneumoniae | adenine + S-ribosyl-L-homocysteine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.37 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
2.1 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli | |
4 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae |