Literature summary for 3.2.2.9 extracted from

Della Ragione, F.; Porcelli, M.; Carteni-Farina, M.; Zappia, V.
Escherichia coli S-adenosylhomocysteine/5'-methylthioadenosine nucleosidasepurification, substrate specificity and mechanisms of action (1985), Biochem. J., 232, 335-341.

Search for more literature for 3.2.2.9

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-Chloroformycin	powerful inhibitor	Escherichia coli
5'-isobutylthio-3-deaza-adenosine	poor inhibitor	Escherichia coli
5'-Isobutylthioinosine	-	Escherichia coli
5'-methylthio-3-deaza-adenosine	poor inhibitor	Escherichia coli
5'-Methylthioformycin	powerful inhibitor	Escherichia coli
5'-methylthiotubercidin	powerful inhibitor	Escherichia coli
5'-n-butylthioinosine	-	Escherichia coli
adenine	poor inhibitor	Escherichia coli
additional information	not inhibited by 5'-methylthioinosine, S-inosyl-L-homocysteine, S-guanosyl-L-homocysteine, S-N6-methyl-3-deaza-adenosyl-L-homocysteine, S-adenosyl-L-homocysteine dialdehyde, acyclic adenosyl-L-homocysteine, S-cytidylhomocysteine, hypoxanthine, guanine, and AMP	Escherichia coli
S-8-aza-adenosyl-L-homocysteine	powerful inhibitor	Escherichia coli
S-8-aza-adenosylhomocysteine	-	Escherichia coli
S-adenosyl-L-homocysteine sulfoxide	poor inhibitor	Escherichia coli
S-formycinylhomocysteine	powerful inhibitor	Escherichia coli
S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine	poor inhibitor	Escherichia coli
S-Tubercidinylhomocysteine	powerful inhibitor	Escherichia coli
sinefungin	poor inhibitor	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	5'-methylthioadenosine	-	Escherichia coli
0.0043	-	S-adenosylhomocysteine	-	Escherichia coli
0.0043	-	S-adenosyl-L-homocysteine	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5'-methylthioadenosine + H2O	Escherichia coli	-	?	-	?
S-adenosyl-L-homocysteine + H2O	Escherichia coli	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
9800-fold	Escherichia coli
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0152	-	crude extract, with S-adenosyl-L-homocysteine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
156	-	substrate S-adenosyl-L-homocysteine	Escherichia coli
156	-	after 9810fold purification, with S-adenosyl-L-homocysteine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
373	-	substrate 5'-methylthioadenosine	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, 50 mM potassium phosphate buffer (pH 7.0), at least 2 months, no significant loss of activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-isobutylthioadenosine + H2O	rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 52%	Escherichia coli	adenine + 5-isobutylthioribose	-	?
5'-methylthioadenosine + H2O	-	Escherichia coli	5-methylthio-D-ribose + adenine	-	?
5'-methylthioadenosine + H2O	-	Escherichia coli	?	-	?
S-2-aza-adenosylhomocysteine + H2O	rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 27%	Escherichia coli	2-azaadenine + 5-(S-L-homocysteinyl)ribose	-	?
S-adenosyl-D-homocysteine + H2O	rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 26%	Escherichia coli	adenine + 5-(S-D-homocysteinyl)ribose	-	?
S-adenosyl-L-homocysteine + H2O	rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 42%	Escherichia coli	adenine + S-ribosyl-L-homocysteine	-	?
S-adenosyl-L-homocysteine + H2O	-	Escherichia coli	?	-	?
S-adenosyl-L-homocysteine + H2O	42% activity compared to 5'-methylthioadenosine	Escherichia coli	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine	-	?

Synonyms

Synonyms	Comment	Organism
adoHcy/MTA nucleosidase	-	Escherichia coli
S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase	-	Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	-	rapidly inactivated after exposure for 15 min	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	rapidly inactivated after exposure for 15 min	Escherichia coli
60	-	the enzyme is rapidly inactivated after exposure for 15 min at 60°C	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000097	-	S-formycinylhomocysteine	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
0.000027	-	5'-Methylthioformycin	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
0.000322	-	5'-Chloroformycin	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
0.000928	-	S-8-aza-adenosyl-L-homocysteine	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
0.0019	-	S-Tubercidinylhomocysteine	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli
0.0041	-	5'-methylthiotubercidin	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00002	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	S-formycinylhomocysteine
0.00006	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-Methylthioformycin
0.0004	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-Chloroformycin
0.0018	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	S-8-aza-adenosyl-L-homocysteine
0.0032	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	S-Tubercidinylhomocysteine
0.0077	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-methylthiotubercidin
0.013	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-isobutylthio-3-deaza-adenosine
0.02	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine
0.024	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	sinefungin
0.069	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	S-adenosyl-L-homocysteine sulfoxide
0.079	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-methylthio-3-deaza-adenosine
0.094	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-n-butylthioinosine
0.132	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	5'-Isobutylthioinosine
0.216	-	in 50 mM potassium phosphate buffer, pH 7.0, at 37°C	Escherichia coli	adenine

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Release 2023.1 (January 2023)