General Stability | Organism |
---|---|
unstable in solution of low ionic strength | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
5'-methylthioadenosine | - |
Escherichia coli | |
9 | - |
S-adenosylhomocysteine | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? | |
5'-methylthioadenosine + H2O | Escherichia coli | - |
? | - |
? | |
5'-methylthioadenosine + H2O | Klebsiella aerogenes | - |
? | - |
? | |
5'-methylthioadenosine + H2O | Escherichia coli B / ATCC 11303 | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Escherichia coli | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Klebsiella aerogenes | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Escherichia coli B / ATCC 11303 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Klebsiella aerogenes | - |
NRRL No. 199 | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
160-fold | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.75 | - |
substrate S-adenosyl-L-homocysteine | Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, loses 50% activity in 48 h, -20°C very unstable, lost nearly all activity when stored for 1 week | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Klebsiella aerogenes | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli B / ATCC 11303 | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Klebsiella aerogenes | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli B / ATCC 11303 | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
S-adenosylmethionine + H2O | - |
Klebsiella aerogenes | adenine + hypoxanthine + methylthioribose + homoserine lactone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nucleosidase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimal pH for both natural substrates, 5'-methylthioadenosine and S-adenosyl-L-homocysteine, with little variation from pH 5.7-7.1 | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | - |
Escherichia coli |