BRENDA - Enzyme Database
show all sequences of 3.2.2.8

Thermodynamics, functional and structural characterization of inosine-uridine nucleoside hydrolase from Leishmania braziliensis

Dalberto, P.; Martinelli, L.; Bachega, J.; Timmers, L.; Pinto, A.; Dadda, A.; Petersen, G.; Subtil, F.; Galina, L.; Villela, A.; Pissinate, K.; Machado, P.; Bizarro, C.; De Souza, O.; De Carvalho Filho, E.; Basso, L.; Santos, D.; RSC Adv. 7, 48861-48875 (2017)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Leishmania braziliensis
Crystallization (Commentary)
Crystallization
Organism
to 1.53 A resolution, orthorhombic space group I222
Leishmania braziliensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
0.5
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
0.6
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.7
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
3.6
-
uridine
positive homotropic cooperativity, Hill coefficient value of 2.4, pH 7.5, 25°C
Leishmania braziliensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
136000
-
gel filtration
Leishmania braziliensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leishmania braziliensis
A4H9Q9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl beta-D-ribofuranoside + H2O
-
752168
Leishmania braziliensis
4-nitrophenol + D-ribose
-
-
-
?
adenosine + H2O
-
752168
Leishmania braziliensis
adenine + D-ribose
-
-
-
?
cytidine + H2O
-
752168
Leishmania braziliensis
cytosine + D-ribose
-
-
-
?
guanosine + H2O
-
752168
Leishmania braziliensis
guanine + D-ribose
-
-
-
?
inosine + H2O
-
752168
Leishmania braziliensis
D-ribose + hypoxanthine
-
-
-
?
uridine + H2O
-
752168
Leishmania braziliensis
uracil + D-ribose
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 34000, SDS-PAGE, 4 * 34340, calculated from sequence
Leishmania braziliensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.039
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.066
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
5.1
-
uridine
pH 7.5, 25°C
Leishmania braziliensis
11.2
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
344
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Leishmania braziliensis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
to 1.53 A resolution, orthorhombic space group I222
Leishmania braziliensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
0.5
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
0.6
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.7
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
3.6
-
uridine
positive homotropic cooperativity, Hill coefficient value of 2.4, pH 7.5, 25°C
Leishmania braziliensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
136000
-
gel filtration
Leishmania braziliensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl beta-D-ribofuranoside + H2O
-
752168
Leishmania braziliensis
4-nitrophenol + D-ribose
-
-
-
?
adenosine + H2O
-
752168
Leishmania braziliensis
adenine + D-ribose
-
-
-
?
cytidine + H2O
-
752168
Leishmania braziliensis
cytosine + D-ribose
-
-
-
?
guanosine + H2O
-
752168
Leishmania braziliensis
guanine + D-ribose
-
-
-
?
inosine + H2O
-
752168
Leishmania braziliensis
D-ribose + hypoxanthine
-
-
-
?
uridine + H2O
-
752168
Leishmania braziliensis
uracil + D-ribose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 34000, SDS-PAGE, 4 * 34340, calculated from sequence
Leishmania braziliensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.039
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.066
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
5.1
-
uridine
pH 7.5, 25°C
Leishmania braziliensis
11.2
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
344
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.047
-
guanosine
pH 7.5, 25°C
Leishmania braziliensis
0.065
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.094
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
22
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
1300
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.047
-
guanosine
pH 7.5, 25°C
Leishmania braziliensis
0.065
-
adenosine
pH 7.5, 25°C
Leishmania braziliensis
0.094
-
cytidine
pH 7.5, 25°C
Leishmania braziliensis
22
-
Inosine
pH 7.5, 25°C
Leishmania braziliensis
1300
-
4-nitrophenyl beta-D-ribofuranoside
pH 7.5, 25°C
Leishmania braziliensis
Other publictions for EC 3.2.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751203
Lenz
Structural explanation for th ...
Escherichia coli
J. Comput. Aided Mol. Des.
32
1375-1388
2018
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751391
Fan
QM/MM and MM MD Simulations o ...
Escherichia coli
J. Phys. Chem. B
122
1121-1131
2018
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752168
Dalberto
-
Thermodynamics, functional an ...
Leishmania braziliensis
RSC Adv.
7
48861-48875
2017
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1
1
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5
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1
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1
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1
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5
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1
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1
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5
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1
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6
1
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5
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5
5
724342
Minici
New determinants in the cataly ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
51
4590-4599
2012
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1
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726441
Porcelli
Thermal unfolding of nucleosid ...
Saccharolobus solfataricus
Protein Pept. Lett.
19
369-374
2012
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1
3
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716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
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1
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2
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707897
Garau
Active site plasticity reveale ...
Escherichia coli
BMC Struct. Biol.
10
14
2010
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1
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1
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1
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4
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2
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1
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1
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1
1
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700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
Plant Cell
21
876-891
2009
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1
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1
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6
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11
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1
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6
1
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8
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2
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1
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7
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1
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9
1
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8
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679803
Porcelli
Pyrimidine-specific ribonucleo ...
Saccharolobus solfataricus
FEBS J.
275
1900-1914
2008
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2
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1
3
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6
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1
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1
1
1
2
2
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1
3
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1
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3
1
1
1
2
2
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1
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696258
Iovane
Structural basis for substrate ...
Escherichia coli
Biochemistry
47
4418-4426
2008
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1
1
10
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18
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1
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2
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2
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18
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1
10
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18
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18
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664140
Muzzolini
New insight in to the mechanis ...
Escherichia coli K-12
Biochemistry
45
773-782
2006
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1
1
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6
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3
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1
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1
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1
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1
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1
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1
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670166
Kim
Genes encoding ribonucleoside ...
Corynebacterium ammoniagenes
Microbiology
152
1169-1177
2006
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654131
Giabbai
Cloning, purification, crystal ...
Escherichia coli
Acta Crystallogr. Sect. D
D60
524-527
2004
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1
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657373
Giabbai
Crystal structure to 1.7 ANG o ...
Escherichia coli
Structure
12
739-749
2004
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1
1
2
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8
2
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3
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1
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1
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1
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8
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663495
Giabbai
Cloning, purification, crystal ...
Escherichia coli K-12
Acta Crystallogr. Sect. D
60
524-527
2004
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1
1
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137114
Allam
-
Nature of enzymes that catalyz ...
Penicillium chrysogenum
Biochem. Syst. Ecol.
15
515-517
1987
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5
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1
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1
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1
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137115
Krenitsky
-
Purine salvage enzymes in man ...
Leishmania donovani
Adv. Exp. Med. Biol.
122
51-56
1980
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1
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1
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1
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7
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1
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1
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7
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137116
Koszalka
Nucleosidases from Leishmania ...
Leishmania donovani
J. Biol. Chem.
254
8185-8193
1979
-
-
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7
6
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1
1
-
1
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1
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1
1
1
10
-
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1
-
1
-
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7
-
6
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1
1
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1
-
1
1
1
10
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
4168
Murray
-
Molecular weight estimations o ...
Pisum sativum
Phytochemistry
10
2645-2648
1971
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137117
Terada
Purification and properties of ...
Pseudomonas fluorescens
J. Biol. Chem.
242
5578-5585
1967
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