BRENDA - Enzyme Database
show all sequences of 3.2.2.8

QM/MM and MM MD Simulations on the pyrimidine-specific nucleoside hydrolase A comprehensive understanding of enzymatic hydrolysis of uridine

Fan, F.; Chen, N.; Wang, Y.; Wu, R.; Cao, Z.; J. Phys. Chem. B 122, 1121-1131 (2018)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
QM/MM simulations. The relatively stronger hydrogen-bond interactions between uridine and the active-site residues Gln227 and Tyr231 play an important role in enhancing the substrate binding and thus promoting the N-glycosidic bond cleavage, in comparison with inosine. The estimated energy barrier is 30 kcal/mol for the hydrolysis of inosine and 22 kcal/mol for uridine. The uridine binding is exothermic by about 23 kcal/mol, and inosine binding by 12 kcal/mol
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
C3T3U2
-
-
Crystallization (Commentary) (protein specific)
Crystallization
Organism
QM/MM simulations. The relatively stronger hydrogen-bond interactions between uridine and the active-site residues Gln227 and Tyr231 play an important role in enhancing the substrate binding and thus promoting the N-glycosidic bond cleavage, in comparison with inosine. The estimated energy barrier is 30 kcal/mol for the hydrolysis of inosine and 22 kcal/mol for uridine. The uridine binding is exothermic by about 23 kcal/mol, and inosine binding by 12 kcal/mol
Escherichia coli
Other publictions for EC 3.2.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751203
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Fan
QM/MM and MM MD Simulations o ...
Escherichia coli
J. Phys. Chem. B
122
1121-1131
2018
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