BRENDA - Enzyme Database
show all sequences of 3.2.2.8

Thermal unfolding of nucleoside hydrolases from the hyperthermophilic archaeon Sulfolobus solfataricus: role of disulfide bonds

Porcelli, M.; De Leo, E.; Del Vecchio, P.; Fuccio, F.; Cacciapuoti, G.; Protein Pept. Lett. 19, 369-374 (2012)

Data extracted from this reference:

Organic Solvent Stability
Organic Solvent
Commentary
Organism
guanidine-HCl
guanidine hydrochloride-induced unfolding is reversible
Saccharolobus solfataricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharolobus solfataricus
-
-
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
108
-
no thermal unfolding transition can be obtained up to 108C
Saccharolobus solfataricus
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
guanidine-HCl
guanidine hydrochloride-induced unfolding is reversible
Saccharolobus solfataricus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
108
-
no thermal unfolding transition can be obtained up to 108C
Saccharolobus solfataricus
Other publictions for EC 3.2.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751203
Lenz
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751391
Fan
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Escherichia coli
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724342
Minici
New determinants in the cataly ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
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726441
Porcelli
Thermal unfolding of nucleosid ...
Saccharolobus solfataricus
Protein Pept. Lett.
19
369-374
2012
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Arabidopsis nucleoside hydrola ...
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700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
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Porcelli
Pyrimidine-specific ribonucleo ...
Saccharolobus solfataricus
FEBS J.
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696258
Iovane
Structural basis for substrate ...
Escherichia coli
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2008
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Muzzolini
New insight in to the mechanis ...
Escherichia coli K-12
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Kim
Genes encoding ribonucleoside ...
Corynebacterium ammoniagenes
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654131
Giabbai
Cloning, purification, crystal ...
Escherichia coli
Acta Crystallogr. Sect. D
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2004
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Giabbai
Crystal structure to 1.7 ANG o ...
Escherichia coli
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2004
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1
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Giabbai
Cloning, purification, crystal ...
Escherichia coli K-12
Acta Crystallogr. Sect. D
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2004
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137114
Allam
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Nature of enzymes that catalyz ...
Penicillium chrysogenum
Biochem. Syst. Ecol.
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1987
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Krenitsky
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Purine salvage enzymes in man ...
Leishmania donovani
Adv. Exp. Med. Biol.
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51-56
1980
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Koszalka
Nucleosidases from Leishmania ...
Leishmania donovani
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10
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7
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6
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10
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1
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4168
Murray
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Molecular weight estimations o ...
Pisum sativum
Phytochemistry
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2645-2648
1971
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16
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