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Literature summary for 3.2.2.8 extracted from

  • Jung, B.; Hoffmann, C.; Moehlmann, T.
    Arabidopsis nucleoside hydrolases involved in intracellular and extracellular degradation of purines (2011), Plant J., 65, 703-711.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
methyluridine isoform NSH1, pH not specified in the publication, at 30°C Arabidopsis thaliana
0.7
-
adenosine isoform NSH1, pH not specified in the publication, at 30°C Arabidopsis thaliana
0.8
-
uridine isoform NSH1, pH not specified in the publication, at 30°C Arabidopsis thaliana
1.4
-
Inosine isoform NSH1, pH not specified in the publication, at 30°C Arabidopsis thaliana
1.69
-
Xanthosine isoform NSH1, pH not specified in the publication, at 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform NSH2 Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyluridine + H2O
-
Arabidopsis thaliana D-ribose + 5-methyluracil
-
?
adenosine + H2O
-
Arabidopsis thaliana adenine + D-ribose
-
?
inosine + H2O
-
Arabidopsis thaliana hypoxanthine + D-ribose
-
?
isopentenyladenine riboside + H2O
-
Arabidopsis thaliana ?
-
?
uridine + H2O
-
Arabidopsis thaliana uracil + D-ribose
-
?
xanthosine + H2O high hydrolytic efficiency, xanthosine is a better substrate for isoform NSH1 compared to inosine and adenosine Arabidopsis thaliana xanthine + D-ribose
-
?

Synonyms

Synonyms Comment Organism
NSH1 former designation URH1 Arabidopsis thaliana
nucleoside hydrolase
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a knockout mutant for NSH1 shows symptoms of accelerated senescence, accompanied by marked accumulation of uridine and xanthosine under conditions of prolonged darkness. Arabidopsis thaliana
physiological function isoform NSH1 represents the leading activity for both pyrimidine and purine nucleoside hydrolysis. Isoform NSH3 functions as an extracellular, purine-specific hydrolase that is involved in degradation of extracellular nucleosides and may participate in wound and pathogen responses. Isoform NSH2 acts during the late phase of senescence and may support inosine breakdown Arabidopsis thaliana