KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
methyluridine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
0.7 | - |
adenosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
0.8 | - |
uridine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
1.4 | - |
Inosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
1.69 | - |
Xanthosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform NSH2 | Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyluridine + H2O | - |
Arabidopsis thaliana | D-ribose + 5-methyluracil | - |
? | |
adenosine + H2O | - |
Arabidopsis thaliana | adenine + D-ribose | - |
? | |
inosine + H2O | - |
Arabidopsis thaliana | hypoxanthine + D-ribose | - |
? | |
isopentenyladenine riboside + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
uridine + H2O | - |
Arabidopsis thaliana | uracil + D-ribose | - |
? | |
xanthosine + H2O | high hydrolytic efficiency, xanthosine is a better substrate for isoform NSH1 compared to inosine and adenosine | Arabidopsis thaliana | xanthine + D-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NSH1 | former designation URH1 | Arabidopsis thaliana |
nucleoside hydrolase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | a knockout mutant for NSH1 shows symptoms of accelerated senescence, accompanied by marked accumulation of uridine and xanthosine under conditions of prolonged darkness. | Arabidopsis thaliana |
physiological function | isoform NSH1 represents the leading activity for both pyrimidine and purine nucleoside hydrolysis. Isoform NSH3 functions as an extracellular, purine-specific hydrolase that is involved in degradation of extracellular nucleosides and may participate in wound and pathogen responses. Isoform NSH2 acts during the late phase of senescence and may support inosine breakdown | Arabidopsis thaliana |