BRENDA - Enzyme Database
show all sequences of 3.2.2.8

Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis

Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.; Plant Cell 21, 876-891 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli and Saccharomyces cerevisiae; URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Arabidopsis thaliana
0.4
-
isopentenyladenine-riboside
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
0.7
-
adenosine
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
0.8
-
uridine
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
1.4
-
Inosine
recombinant enzyme expressed in Escherichia coli; recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
2.1
-
uridine
recombinant enzyme expressed in Saccharomyces cerevisiae, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
Q9SJM7
ecotype Columbia
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
guard cell
;
Arabidopsis thaliana
-
pollen
;
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
root tip
;
Arabidopsis thaliana
-
root vascular cell
URH1 is mainly transcribed in the vascular cells of roots
Arabidopsis thaliana
-
vascular cell
of roots and in root tips
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14.5
-
purified recombinant enzyme, substrate inosine
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine + H2O
-
700741
Arabidopsis thaliana
adenine + D-ribose
-
-
-
?
adenosine + H2O
-
700741
Arabidopsis thaliana
D-ribose + adenine
-
-
-
?
inosine + H2O
-
700741
Arabidopsis thaliana
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
-
700741
Arabidopsis thaliana
D-ribose + hypoxanthine
-
-
-
?
isopentenyladenine riboside + H2O
-
700741
Arabidopsis thaliana
?
-
-
-
?
additional information
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
700741
Arabidopsis thaliana
?
-
-
-
-
uridine + H2O
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
700741
Arabidopsis thaliana
uracil + D-ribose
-
-
-
?
uridine + H2O
-
700741
Arabidopsis thaliana
D-ribose + uracil
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli and Saccharomyces cerevisiae
Arabidopsis thaliana
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Arabidopsis thaliana
0.4
-
isopentenyladenine-riboside
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
0.7
-
adenosine
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
0.8
-
uridine
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
1.4
-
Inosine
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
1.4
-
Inosine
recombinant enzyme expressed in Escherichia coli
Arabidopsis thaliana
2.1
-
uridine
recombinant enzyme expressed in Saccharomyces cerevisiae, in 50 mM Tris-HCl, pH 7.5, at 30C
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
guard cell
-
Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
root tip
-
Arabidopsis thaliana
-
root vascular cell
URH1 is mainly transcribed in the vascular cells of roots
Arabidopsis thaliana
-
vascular cell
of roots and in root tips
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14.5
-
purified recombinant enzyme, substrate inosine
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine + H2O
-
700741
Arabidopsis thaliana
adenine + D-ribose
-
-
-
?
adenosine + H2O
-
700741
Arabidopsis thaliana
D-ribose + adenine
-
-
-
?
inosine + H2O
-
700741
Arabidopsis thaliana
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
-
700741
Arabidopsis thaliana
D-ribose + hypoxanthine
-
-
-
?
isopentenyladenine riboside + H2O
-
700741
Arabidopsis thaliana
?
-
-
-
?
additional information
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
700741
Arabidopsis thaliana
?
-
-
-
-
uridine + H2O
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
700741
Arabidopsis thaliana
uracil + D-ribose
-
-
-
?
uridine + H2O
-
700741
Arabidopsis thaliana
D-ribose + uracil
-
-
-
?
Other publictions for EC 3.2.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751203
Lenz
Structural explanation for th ...
Escherichia coli
J. Comput. Aided Mol. Des.
32
1375-1388
2018
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1
1
1
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3
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751391
Fan
QM/MM and MM MD Simulations o ...
Escherichia coli
J. Phys. Chem. B
122
1121-1131
2018
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1
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1
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752168
Dalberto
-
Thermodynamics, functional an ...
Leishmania braziliensis
RSC Adv.
7
48861-48875
2017
-
-
1
1
-
-
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5
-
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1
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1
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6
1
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5
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1
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1
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5
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1
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6
1
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5
-
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-
5
5
724342
Minici
New determinants in the cataly ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
51
4590-4599
2012
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1
1
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1
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2
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1
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2
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1
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1
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2
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726441
Porcelli
Thermal unfolding of nucleosid ...
Saccharolobus solfataricus
Protein Pept. Lett.
19
369-374
2012
-
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-
-
-
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1
3
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1
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1
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716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
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5
1
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3
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6
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1
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6
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2
2
-
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-
707897
Garau
Active site plasticity reveale ...
Escherichia coli
BMC Struct. Biol.
10
14
2010
-
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1
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1
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1
-
1
-
4
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2
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1
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1
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1
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1
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1
1
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1
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1
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2
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1
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-
1
-
-
-
-
1
1
-
-
-
700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
Plant Cell
21
876-891
2009
-
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1
-
1
-
-
6
-
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-
-
-
11
-
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1
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6
1
-
8
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2
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1
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7
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1
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9
1
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8
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679803
Porcelli
Pyrimidine-specific ribonucleo ...
Saccharolobus solfataricus
FEBS J.
275
1900-1914
2008
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1
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2
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1
3
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6
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1
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3
1
1
1
2
2
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1
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1
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2
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1
3
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1
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3
1
1
1
2
2
-
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-
1
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-
-
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696258
Iovane
Structural basis for substrate ...
Escherichia coli
Biochemistry
47
4418-4426
2008
-
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1
1
10
-
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18
-
1
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2
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1
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2
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18
-
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1
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1
10
-
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18
-
1
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1
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2
-
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18
-
-
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664140
Muzzolini
New insight in to the mechanis ...
Escherichia coli K-12
Biochemistry
45
773-782
2006
-
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1
1
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-
6
-
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-
3
-
1
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1
1
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9
1
1
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6
1
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1
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1
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6
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1
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9
1
1
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6
1
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670166
Kim
Genes encoding ribonucleoside ...
Corynebacterium ammoniagenes
Microbiology
152
1169-1177
2006
-
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1
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3
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2
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654131
Giabbai
Cloning, purification, crystal ...
Escherichia coli
Acta Crystallogr. Sect. D
D60
524-527
2004
-
-
1
1
-
-
-
-
-
-
2
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3
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1
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1
1
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1
1
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657373
Giabbai
Crystal structure to 1.7 ANG o ...
Escherichia coli
Structure
12
739-749
2004
-
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1
1
2
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8
2
-
3
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4
-
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1
1
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7
1
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8
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1
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1
2
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8
2
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3
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1
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7
1
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8
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663495
Giabbai
Cloning, purification, crystal ...
Escherichia coli K-12
Acta Crystallogr. Sect. D
60
524-527
2004
-
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1
1
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2
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3
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1
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4
2
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1
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4
2
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137114
Allam
-
Nature of enzymes that catalyz ...
Penicillium chrysogenum
Biochem. Syst. Ecol.
15
515-517
1987
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5
-
1
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1
-
1
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137115
Krenitsky
-
Purine salvage enzymes in man ...
Leishmania donovani
Adv. Exp. Med. Biol.
122
51-56
1980
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1
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1
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1
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7
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1
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1
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7
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137116
Koszalka
Nucleosidases from Leishmania ...
Leishmania donovani
J. Biol. Chem.
254
8185-8193
1979
-
-
-
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7
6
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1
1
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1
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1
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1
1
1
10
-
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1
-
1
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7
-
6
-
-
1
1
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-
1
-
1
1
1
10
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
4168
Murray
-
Molecular weight estimations o ...
Pisum sativum
Phytochemistry
10
2645-2648
1971
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1
1
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1
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1
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1
1
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1
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3
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137117
Terada
Purification and properties of ...
Pseudomonas fluorescens
J. Biol. Chem.
242
5578-5585
1967
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