Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and Saccharomyces cerevisiae | Arabidopsis thaliana |
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.4 | - |
isopentenyladenine-riboside | recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C | Arabidopsis thaliana | |
0.7 | - |
adenosine | recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C | Arabidopsis thaliana | |
0.8 | - |
uridine | recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C | Arabidopsis thaliana | |
1.4 | - |
Inosine | recombinant enzyme expressed in Escherichia coli | Arabidopsis thaliana | |
1.4 | - |
Inosine | recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C | Arabidopsis thaliana | |
2.1 | - |
uridine | recombinant enzyme expressed in Saccharomyces cerevisiae, in 50 mM Tris-HCl, pH 7.5, at 30°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9SJM7 | ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
guard cell | - |
Arabidopsis thaliana | - |
pollen | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
root tip | - |
Arabidopsis thaliana | - |
root vascular cell | URH1 is mainly transcribed in the vascular cells of roots | Arabidopsis thaliana | - |
vascular cell | of roots and in root tips | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.5 | - |
purified recombinant enzyme, substrate inosine | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Arabidopsis thaliana | adenine + D-ribose | - |
? | |
adenosine + H2O | - |
Arabidopsis thaliana | D-ribose + adenine | - |
? | |
inosine + H2O | - |
Arabidopsis thaliana | hypoxanthine + D-ribose | - |
? | |
inosine + H2O | - |
Arabidopsis thaliana | D-ribose + hypoxanthine | - |
? | |
isopentenyladenine riboside + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine | Arabidopsis thaliana | ? | - |
? | |
uridine + H2O | the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine | Arabidopsis thaliana | uracil + D-ribose | - |
? | |
uridine + H2O | - |
Arabidopsis thaliana | D-ribose + uracil | - |
? |
Synonyms | Comment | Organism |
---|---|---|
URH1 | URH1 is a member of the inosine-uridine nucleosidase protein family | Arabidopsis thaliana |
uridine-ribohydrolase 1 | - |
Arabidopsis thaliana |