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Literature summary for 3.2.2.8 extracted from

  • Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.
    Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis (2009), Plant Cell, 21, 876-891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Saccharomyces cerevisiae Arabidopsis thaliana
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana
0.4
-
isopentenyladenine-riboside recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
0.7
-
adenosine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
0.8
-
uridine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
1.4
-
Inosine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
1.4
-
Inosine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
2.1
-
uridine recombinant enzyme expressed in Saccharomyces cerevisiae, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q9SJM7 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
guard cell
-
Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
root tip
-
Arabidopsis thaliana
-
root vascular cell URH1 is mainly transcribed in the vascular cells of roots Arabidopsis thaliana
-
vascular cell of roots and in root tips Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.5
-
purified recombinant enzyme, substrate inosine Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
Arabidopsis thaliana adenine + D-ribose
-
?
adenosine + H2O
-
Arabidopsis thaliana D-ribose + adenine
-
?
inosine + H2O
-
Arabidopsis thaliana hypoxanthine + D-ribose
-
?
inosine + H2O
-
Arabidopsis thaliana D-ribose + hypoxanthine
-
?
isopentenyladenine riboside + H2O
-
Arabidopsis thaliana ?
-
?
additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
?
uridine + H2O the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana uracil + D-ribose
-
?
uridine + H2O
-
Arabidopsis thaliana D-ribose + uracil
-
?

Synonyms

Synonyms Comment Organism
URH1 URH1 is a member of the inosine-uridine nucleosidase protein family Arabidopsis thaliana
uridine-ribohydrolase 1
-
Arabidopsis thaliana