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Literature summary for 3.2.2.8 extracted from

  • Porcelli, M.; Concilio, L.; Peluso, I.; Marabotti, A.; Facchiano, A.; Cacciapuoti, G.
    Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus - biochemical characterization and homology modeling (2008), FEBS J., 275, 1900-1914.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
uridine pH 7.4, 80°C Saccharolobus solfataricus
0.97
-
cytidine pH 7.4, 80°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required in maintaining the active site structure Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Saccharolobus solfataricus
35210
-
4 * 35210, calculated from sequence Saccharolobus solfataricus
140000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97ZS5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine + H2O
-
Saccharolobus solfataricus cytosine + D-ribose
-
?
inosine + H2O catalytic efficiency towards inosine is at least 100-fold below that for uridine Saccharolobus solfataricus ?
-
?
uridine + H2O
-
Saccharolobus solfataricus uracil + D-ribose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Saccharolobus solfataricus
tetramer 4 * 35210, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SsCU-NH
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 110 60°C: about 50% of maximal activity, 110°C: about 60% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
1 h, stable Saccharolobus solfataricus
100
-
half-life: 37 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1
-
uridine pH 7.4, 80°C Saccharolobus solfataricus
39.4
-
cytidine pH 7.4, 80°C Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus calculated from sequence
-
5.2