Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
uridine | pH 7.4, 80°C | Saccharolobus solfataricus | |
0.97 | - |
cytidine | pH 7.4, 80°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required in maintaining the active site structure | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Saccharolobus solfataricus |
35210 | - |
4 * 35210, calculated from sequence | Saccharolobus solfataricus |
140000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97ZS5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine + H2O | - |
Saccharolobus solfataricus | cytosine + D-ribose | - |
? | |
inosine + H2O | catalytic efficiency towards inosine is at least 100-fold below that for uridine | Saccharolobus solfataricus | ? | - |
? | |
uridine + H2O | - |
Saccharolobus solfataricus | uracil + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Saccharolobus solfataricus |
tetramer | 4 * 35210, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SsCU-NH | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 110 | 60°C: about 50% of maximal activity, 110°C: about 60% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
1 h, stable | Saccharolobus solfataricus |
100 | - |
half-life: 37 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
uridine | pH 7.4, 80°C | Saccharolobus solfataricus | |
39.4 | - |
cytidine | pH 7.4, 80°C | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | calculated from sequence | - |
5.2 |