Cloned (Comment) | Organism |
---|---|
gene ybeK, expression in strain W6 | Escherichia coli K-12 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with D-ribose, hanging drop vapour diffusion method, 10 mg/ml protein in 10 mM Tris, pH 7.0, 25 mM NaCl, and 500 mM D-ribose, is mixed with an equal volume of precipitant solution containing 24% 2-methyl-2,4-pentanediol, 0.1 M sodium acetate, pH 5.0, and 500 mM D-ribose, 20°C, 1 week, X-ray diffraction structure determination and analysis at 1.78 A resolution | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
uridine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.18 | - |
4-nitrophenyl ribopyranoside | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.239 | - |
Inosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.363 | - |
guanosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.524 | - |
cytidine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.648 | - |
adenosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli K-12 | the enzyme is required for recycling of nitrogenous bases | ? | - |
? | |
N-ribosylpurine + H2O | Escherichia coli K-12 | - |
purine + D-ribose | - |
r | |
N-ribosylpyrimidine + H2O | Escherichia coli K-12 | preferred substrate | pyrimidine + D-ribose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P33022 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain W6 by nickel chelate affinity chromatography and gel filtration | Escherichia coli K-12 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base | catalytic mechanism, active site structure, conformational transition between open and closed structure, product release mechanism | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl ribopyranoside + H2O | - |
Escherichia coli K-12 | 4-nitrophenol + D-ribose | - |
? | |
adenosine + H2O | - |
Escherichia coli K-12 | adenine + D-ribose | - |
? | |
cytidine + H2O | - |
Escherichia coli K-12 | cytosine + D-ribose | - |
? | |
guanosine + H2O | - |
Escherichia coli K-12 | guanine + D-ribose | - |
? | |
inosine + H2O | - |
Escherichia coli K-12 | hypoxanthine + D-ribose | - |
? | |
additional information | the enzyme is required for recycling of nitrogenous bases | Escherichia coli K-12 | ? | - |
? | |
N-ribosylpurine + H2O | - |
Escherichia coli K-12 | purine + D-ribose | - |
r | |
N-ribosylpyrimidine + H2O | preferred substrate | Escherichia coli K-12 | pyrimidine + D-ribose | - |
r | |
uridine + H2O | - |
Escherichia coli K-12 | uracil + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | determination of the quarternary structure | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
NH | - |
Escherichia coli K-12 |
nucleoside hydrolase | - |
Escherichia coli K-12 |
RihA | - |
Escherichia coli K-12 |
YbeK | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
guanosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.0154 | - |
adenosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
0.043 | - |
Inosine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
11.6 | - |
cytidine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
13.9 | - |
uridine | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 | |
39 | - |
4-nitrophenyl ribopyranoside | pH 7.0, 35°C, recombinant enzyme | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli K-12 |