Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H239A | dramatic increase in Km for uridine, unchanged kcat | Escherichia coli |
H82N | small increase in Km, increase in kcat | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
5-fluorouridine | pH 7.3, 37°C | Escherichia coli | |
0.142 | - |
uridine | pH 7.3, 37°C | Escherichia coli | |
0.186 | - |
5-Bromouridine | pH 7.3, 37°C | Escherichia coli | |
0.22 | - |
5-iodouridine | pH 7.3, 37°C | Escherichia coli | |
0.329 | - |
5-methyluridine | pH 7.3, 37°C | Escherichia coli | |
0.345 | - |
uridine | pH 7.3, 37°C, H82N mutant | Escherichia coli | |
0.532 | - |
cytidine | pH 7.3, 37°C | Escherichia coli | |
1.9 | - |
uridine | pH 7.3, 37°C, H239A mutant | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
homotetramer, 4 * 33000, SDS-PAGE | Escherichia coli |
137000 | - |
calculated from amino acid sequence | Escherichia coli |
143000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromouridine + H2O | - |
Escherichia coli | 5-bromouracil + D-ribose | - |
? | |
5-fluorouridine + H2O | - |
Escherichia coli | 5-fluorouracil + D-ribose | - |
? | |
5-iodouridine + H2O | - |
Escherichia coli | 5-iodouracil + D-ribose | - |
? | |
5-methyluridine + H2O | - |
Escherichia coli | 5-methyluracil + D-ribose | - |
? | |
cytidine + H2O | - |
Escherichia coli | cytosine + D-ribose | - |
? | |
additional information | no substrate: deazouridine | Escherichia coli | ? | - |
? | |
uridine + H2O | - |
Escherichia coli | uracil + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | homotetramer, 4 * 33000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
YeiK | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
uridine | pH 7.3, 37°C, H239A mutant | Escherichia coli | |
4.7 | - |
uridine | pH 7.3, 37°C | Escherichia coli | |
11.6 | - |
cytidine | pH 7.3, 37°C | Escherichia coli | |
14.7 | - |
5-fluorouridine | pH 7.3, 37°C | Escherichia coli | |
15.5 | - |
uridine | pH 7.3, 37°C, H82N mutant | Escherichia coli | |
25.5 | - |
5-methyluridine | pH 7.3, 37°C | Escherichia coli | |
30.8 | - |
5-Bromouridine | pH 7.3, 37°C | Escherichia coli | |
42.7 | - |
5-iodouridine | pH 7.3, 37°C | Escherichia coli |