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Literature summary for 3.2.2.8 extracted from

  • Terada, M.; Tatibana, M.; Hayaishi, O.
    Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens (1967), J. Biol. Chem., 242, 5578-5585.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
adenosine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
AgCl at 1 mM: inhibition less than 10% Pseudomonas fluorescens
CaCl2 at 1 mM, inhibition less than 10% Pseudomonas fluorescens
CdCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
CoCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
CuCl2 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
cytidine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
EDTA at 1 mM: inhibits uridine hydrolysis by 50% Pseudomonas fluorescens
FeCl3 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
FeSO4 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
HgCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
Inosine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
MgCl2 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
MnSO4 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
NH4Cl at 1 mM: inhibition less than 10% Pseudomonas fluorescens
ZnCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
Inosine pH 8.5 Pseudomonas fluorescens
0.5
-
Inosine
-
Pseudomonas fluorescens
0.5
-
adenosine pH 8.5 Pseudomonas fluorescens
0.6
-
guanosine
-
Pseudomonas fluorescens
0.8
-
uridine
-
Pseudomonas fluorescens
1
-
cytidine pH 8.5 Pseudomonas fluorescens
2.5
-
5-Bromouridine
-
Pseudomonas fluorescens
6.5
-
Xanthosine
-
Pseudomonas fluorescens
7.5
-
1-beta-D-ribofuranosylthymine
-
Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-D-ribosylpyrimidine Pseudomonas fluorescens
-
pyrimidine + D-ribose
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, 300fold Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
272
-
at 37°C Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-15°C, 1-3 mg of protein per ml, stable for 6 months Pseudomonas fluorescens
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-beta-D-ribofuranosylnicotinamide + H2O
-
Pseudomonas fluorescens nicotinamide + D-ribose
-
?
1-beta-D-ribofuranosylthymine + H2O
-
Pseudomonas fluorescens D-ribose + thymine
-
?
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
Pseudomonas fluorescens 4-amino-5-imidazolecarboxamide + D-ribose
-
?
5-bromouridine + H2O
-
Pseudomonas fluorescens 5-bromouracil + D-ribose
-
?
6-azauridine + H2O poor substrate Pseudomonas fluorescens 6-azauracil + D-ribose
-
?
adenosine + H2O
-
Pseudomonas fluorescens adenine + D-ribose
-
?
cytidine + H2O
-
Pseudomonas fluorescens cytosine + D-ribose
-
?
guanosine + H2O
-
Pseudomonas fluorescens guanine + D-ribose
-
?
imidazoleacetic acid ribonucleotide + H2O
-
Pseudomonas fluorescens imidazoleacetic acid + D-ribose
-
?
inosine + H2O
-
Pseudomonas fluorescens hypoxanthine + D-ribose
-
?
N-D-ribosylpyrimidine
-
Pseudomonas fluorescens pyrimidine + D-ribose
-
?
N-D-ribosylpyrimidine + H2O
-
Pseudomonas fluorescens pyrimidine + D-ribose
-
?
purine D-ribonucleosides + H2O prefers pyrimidines Pseudomonas fluorescens purine + D-ribose
-
?
tubercidin + H2O poor substrate Pseudomonas fluorescens 1-deazaadenine + D-ribose
-
?
uridine + H2O
-
Pseudomonas fluorescens uracil + D-ribose
-
?
xanthosine + H2O
-
Pseudomonas fluorescens xanthine + D-ribose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
for 10 min at pH 7.0-9.2, more than 90% of activity remains Pseudomonas fluorescens
45
-
for 10 min at pH 5.0, 50% of activity remains Pseudomonas fluorescens
60
-
for 15 min, 60% denaturation Pseudomonas fluorescens
70
-
for 5 min at pH 8.0, complete inactivation Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
substrate: inosine Pseudomonas fluorescens
6.5
-
substrate: guanosine Pseudomonas fluorescens
7
-
substrate: xanthosine Pseudomonas fluorescens
8
-
substrate: 1-beta-D-ribofuranosylthymine Pseudomonas fluorescens
8.5
-
substrate: cytidine, uridine, 5-bromouridine and adenosine Pseudomonas fluorescens