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Literature summary for 3.2.2.8 extracted from

  • Koszalka, G.W.; Krenitsky, T.A.
    Nucleosidases from Leishmania donovani. Pyrimidine ribonucleosidase, purine ribonucleosidase, and a novel purine 2-deoxyribonucleosidase (1979), J. Biol. Chem., 254, 8185-8193.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4,6-Dihydroxy-1-beta-D-ribofuranosylpyrazolo-(3,4-d) pyrimidine
-
Leishmania donovani
4-Hydroxyl-1-beta-D-ribofuranosylpyrazolo-(3,4-d) pyrimidine
-
Leishmania donovani
4-Methylthio-1-beta-D-ribofuranosylpyrazolo-(3,4-d)pyrimidine
-
Leishmania donovani
4-Thio-1-beta-D-ribofuranosylpyrazolo-(3,4-d)pyrimidine
-
Leishmania donovani
adenosine
-
Leishmania donovani
guanosine
-
Leishmania donovani
additional information poor inhibitors Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
6-mercaptopurine ribonucleoside
-
Leishmania donovani
0.62
-
Inosine
-
Leishmania donovani
0.78
-
purine ribonucleoside
-
Leishmania donovani
1.06
-
uridine
-
Leishmania donovani
1.11
-
Xanthosine
-
Leishmania donovani
5.5
-
uridine
-
Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-D-ribosylpyrimidine Leishmania donovani
-
pyrimidine + D-ribose
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania donovani
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
substrate: uridine Leishmania donovani

Storage Stability

Storage Stability Organism
3°C, cell extract heated for 10 min at 55°C before passage through a DEAE-cellulose column, enzyme thus purified loses 5% of activity after 30 days Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-mercaptopurine ribonucleoside + H2O
-
Leishmania donovani 6-mercaptopurine + D-ribose
-
?
cytidine + H2O
-
Leishmania donovani cytosine + D-ribose
-
?
inosine + H2O
-
Leishmania donovani hypoxanthine + D-ribose
-
?
additional information not: adenosine and guanosine Leishmania donovani ?
-
?
N-D-ribosylpyrimidine
-
Leishmania donovani pyrimidine + D-ribose
-
?
N-D-ribosylpyrimidine + H2O
-
Leishmania donovani pyrimidine + D-ribose
-
?
purine D-ribonucleosides + H2O appreciable substrate activity if a hydroxyl or thio group is present in the 6-position or if a hydroxyl group is present in the 2- and 6-position, preference for oxo versus amino substituents Leishmania donovani purine + D-ribose
-
?
purine D-ribonucleosides + H2O prefers pyrimidines Leishmania donovani purine + D-ribose
-
?
uridine + H2O cleaves uridine most efficiantly Leishmania donovani uracil + D-ribose
-
?
xanthosine + H2O
-
Leishmania donovani xanthine + D-ribose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
after 2 min: all of the uridine-cleaving activity and 62% of the inosine-cleaving activity remains Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
substrate: uridine Leishmania donovani