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Literature summary for 3.2.2.7 extracted from

  • Clark, M.C.; Page, O.T.; Fisher, M.G.
    Purification and properties of N-ribosyladenine ribohydrolase from potato leaves (1972), Phytochemistry, 11, 3413-3419.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
adenine 64% inhibition at 4 mM Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62400
-
sucrose density gradient centrifugation, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Solanum tuberosum hydrolysis at the same rate as for adenosine 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O Solanum tuberosum 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O Solanum tuberosum 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
ir

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.6
-
-
Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O hydrolysis at the same rate as for adenosine Solanum tuberosum 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Solanum tuberosum 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Solanum tuberosum 6-aminopurine + D-ribofuranose
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 in phosphate buffer Solanum tuberosum
4
-
in acetate buffer Solanum tuberosum