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Literature summary for 3.2.2.7 extracted from

  • Imagawa, H.; Yamano, H.; Inoue, K.; Takino, Y.
    Purification and properties of adenosine nucleosidases from tea leaves (1979), Agric. Biol. Chem., 43, 2337-2342.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
adenine enzyme II inhibited at 0.2 mM, enzymes I and III not inhibited up to 1 mM Camellia sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
for enzymes I, II and III Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Camellia sinensis 3-4 times greater hydrolysis compared to adenosine 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O Camellia sinensis 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O Camellia sinensis component of the purine salvage pathway 6-aminopurine + D-ribofuranose
-
?
adenosine + H2O Camellia sinensis 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
?

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
tea
-

Purification (Commentary)

Purification (Comment) Organism
partial, three forms isolated: enzymes I, II and III Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Camellia sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
for enzyme I Camellia sinensis
12.84
-
for enzyme II Camellia sinensis
7.97
-
for enzyme III Camellia sinensis

Storage Stability

Storage Stability Organism
-20°C, one month, gradually loss of activity on storage at 4°C Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O 3-4 times greater hydrolysis compared to adenosine Camellia sinensis 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Camellia sinensis 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O component of the purine salvage pathway Camellia sinensis 6-aminopurine + D-ribofuranose
-
?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Camellia sinensis 6-aminopurine + D-ribofuranose
-
?
6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O rapid hydrolysis Camellia sinensis 6-aminopurine-N1-oxide + D-ribofuranose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
for enzymes II and III Camellia sinensis
45
-
for enzyme I Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
rapid loss of activity, enzymes I, II and III Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
for enzymes I and III Camellia sinensis
4.5
-
for enzyme II Camellia sinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable within, enzymes I, II and III Camellia sinensis