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Literature summary for 3.2.2.7 extracted from

  • Chen, C.M.; Kristopeit, S.M.
    Metabolism of cytokinin: Deribosylation of cytokinin ribonucleoside by adenosine nucleosidase from wheat germ cells (1981), Plant Physiol., 68, 1020-1023.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.7

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine one third inhibition at 0.5 mM Triticum aestivum
adenosine 78% inhibition at 0.5 mM Triticum aestivum
N6-Benzyladenosine 53% inhibition at 0.5 mM Triticum aestivum
N6-furfuryladenosine 53% inhibition at 0.5 mM Triticum aestivum
trans-6-(4-hydroxy-3-methyl-2-butenylamino)-9-beta-D-ribofuranosylpurine 70% inhibition at 0.5 mM Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 adenosine
-
 Triticum aestivum
0.002
-
 N6-(DELTA2-isopentenyl)-adenosine
-
 Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Triticum aestivum implicated in metabolism of cytokinins 6-aminopurine + D-ribofuranose
-
 ?
adenosine + H2O Triticum aestivum 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.74
-
-
 Triticum aestivum

Storage Stability

Storage Stability Organism
-20°C, 95% retaining activity after 30 days Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O implicated in metabolism of cytokinins Triticum aestivum 6-aminopurine + D-ribofuranose
-
 ?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Triticum aestivum 6-aminopurine + D-ribofuranose
-
 ?
N6-(DELTA2-isopentenyl)-adenosine + H2O N6-(DELTA2-isopentenyl)-6-aminopurine-9-beta-D-ribofuranoside Triticum aestivum N6-(DELTA2-isopentenyl)-6-aminopurine + D-ribofuranose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
-
 Triticum aestivum