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Literature summary for 3.2.2.7 extracted from

  • Burch, L.R.; Stuchbury, T.
    Purification and properties of adenosine nucleosidases from tomato (Lycopersicon esculentum) roots and leaves (1986), J. Plant Physiol., 125, 267-273.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Inosine at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited Solanum lycopersicum
N6-Benzyladenosine 36.5% inhibition for form R1, 1.6% for form R2, form Lf not inhibited at 0.2 mM Solanum lycopersicum
N6-isopentenyladenosine inhibition between 28.6% and 36.7% for forms R1, R2 and Lf at 0.2 mM Solanum lycopersicum
zeatin riboside competitive inhibition between 32% and 25% for forms R1, R2 and Lf at 0.2 mM Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
adenosine from Lf Solanum lycopersicum
0.009
-
adenosine form R2 Solanum lycopersicum
0.025
-
adenosine form R1 Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Solanum lycopersicum component of the purine salvage pathway 6-aminopurine + D-ribofuranose
-
ir
adenosine + H2O Solanum lycopersicum implicated in metabolism of cytokinins 6-aminopurine + D-ribofuranose
-
ir
adenosine + H2O Solanum lycopersicum 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
ir

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
three forms: R1, R2 and Lf
-

Purification (Commentary)

Purification (Comment) Organism
form R2 to homogeneity, forms R1 and Lf partial Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf form Lf Solanum lycopersicum
-
root two forms, R1 and R2 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O component of the purine salvage pathway Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
ir
adenosine + H2O implicated in metabolism of cytokinins Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
ir
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
for form R1 Solanum lycopersicum
6
-
forms R2 and Lf Solanum lycopersicum