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Literature summary for 3.2.2.7 extracted from

  • Abusamhadneh, E.; McDonald, N.E.; Kline, P.C.
    Isolation and characterization of adenosine nucleosidase from yellow lupin (Lupinus luteus) (2000), Plant Sci., 153, 25-32.
No PubMed abstract available

General Stability

General Stability Organism
stable against repeated freezing and thawing cycles Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
adenosine
-
Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
gel filtration Lupinus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Lupinus luteus one third lowered activity compared to adenosine as substrate 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O Lupinus luteus 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O Lupinus luteus component of the purine salvage pathway 6-aminopurine + D-ribofuranose
-
?
adenosine + H2O Lupinus luteus 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol remains active up to 20%, v/v Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
-
Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + H2O 9-ribosylhydroxanthine, low activity Lupinus luteus hypoxanthine + D-ribose
-
?
purine riboside + H2O low activity Lupinus luteus purine + D-ribose
-
?
2'-deoxyadenosine + H2O one third lowered activity compared to adenosine as substrate Lupinus luteus 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Lupinus luteus 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
adenosine + H2O component of the purine salvage pathway Lupinus luteus 6-aminopurine + D-ribofuranose
-
?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Lupinus luteus 6-aminopurine + D-ribofuranose
-
?
5'-deoxyadenosine + H2O 6-aminopurine-9-5'-deoxy-beta-D-ribofuranoside, best substrate Lupinus luteus 6-aminopurine + 5-deoxy-D-ribofuranose
-
?
guanosine + H2O 2-amino-1,9-dihydro-6-oxopurine-9-beta-D-ribofuranoside, two third lowered activity compared to adenosine as substrate Lupinus luteus 2-amino-1,9-dihydropurine-6-on + D-ribofuranose
-
?
6-benzylaminopurine riboside + H2O low activity Lupinus luteus 6-benzylaminopurine + D-ribose
-
?
3'-deoxyadenosine + H2O 6-aminopurine-9-3'-deoxy-beta-D-ribofuranoside, cordycepin, very low activity Lupinus luteus 6-aminopurine + 3-deoxy-D-ribofuranose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33000-36000, SDS-PAGE Lupinus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
adenosine
-
Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Lupinus luteus