BRENDA - Enzyme Database
show all sequences of 3.2.2.6

Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate

Ikeda, T.; Takasawa, S.; Noguchi, N.; Nata, K.; Yamauchi, A.; Takahashi, I.; Yoshikawa, T.; Sugawara, A.; Yonekura, H.; Okamoto, H.; Mol. Cell. Biochem. 366, 69-80 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene cd38, eight exons, DNA and amino acid sequence determination and analysis, sequence comparison to the human enzyme, phylogenetic tree, expression in COS-7 cells
Xenopus laevis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
most frog enzyme (81%) is distributed in the membrane fraction (5% in mitochondrial fraction and 14% in microsomal fraction)
Xenopus laevis
16020
-
microsome
-
Xenopus laevis
-
-
mitochondrion
mitochondrial membrane
Xenopus laevis
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28970
-
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
45000
-
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclic ADP-ribose + H2O
Xenopus laevis
-
ADP-D-ribose
-
-
?
additional information
Xenopus laevis
frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities
?
-
-
-
NAD+
Xenopus laevis
-
cyclic ADP-ribose + nicotinamide
-
-
?
NAD+ + H2O
Xenopus laevis
overall reaction
ADP-D-ribose + nicotinamide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xenopus laevis
H7C831
gene cd38
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Xenopus laevis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Xenopus laevis
-
liver
-
Xenopus laevis
-
additional information
the enzyme is ubiquitously expressed in various tissues
Xenopus laevis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0604
-
NAD+-glycohydrolase activityin crude cell extract, pH 7.4, 37C
Xenopus laevis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclic ADP-ribose + H2O
-
732501
Xenopus laevis
ADP-D-ribose
-
-
-
?
additional information
frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities
732501
Xenopus laevis
?
-
-
-
-
NAD+
-
732501
Xenopus laevis
cyclic ADP-ribose + nicotinamide
-
-
-
?
NAD+ + H2O
overall reaction
732501
Xenopus laevis
ADP-D-ribose + nicotinamide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Xenopus laevis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Xenopus laevis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cd38, eight exons, DNA and amino acid sequence determination and analysis, sequence comparison to the human enzyme, phylogenetic tree, expression in COS-7 cells
Xenopus laevis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
most frog enzyme (81%) is distributed in the membrane fraction (5% in mitochondrial fraction and 14% in microsomal fraction)
Xenopus laevis
16020
-
microsome
-
Xenopus laevis
-
-
mitochondrion
mitochondrial membrane
Xenopus laevis
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28970
-
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
45000
-
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclic ADP-ribose + H2O
Xenopus laevis
-
ADP-D-ribose
-
-
?
additional information
Xenopus laevis
frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities
?
-
-
-
NAD+
Xenopus laevis
-
cyclic ADP-ribose + nicotinamide
-
-
?
NAD+ + H2O
Xenopus laevis
overall reaction
ADP-D-ribose + nicotinamide
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Xenopus laevis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Xenopus laevis
-
liver
-
Xenopus laevis
-
additional information
the enzyme is ubiquitously expressed in various tissues
Xenopus laevis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0604
-
NAD+-glycohydrolase activityin crude cell extract, pH 7.4, 37C
Xenopus laevis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclic ADP-ribose + H2O
-
732501
Xenopus laevis
ADP-D-ribose
-
-
-
?
additional information
frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities
732501
Xenopus laevis
?
-
-
-
-
NAD+
-
732501
Xenopus laevis
cyclic ADP-ribose + nicotinamide
-
-
-
?
NAD+ + H2O
overall reaction
732501
Xenopus laevis
ADP-D-ribose + nicotinamide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE, x * 28970, sequence calculation
Xenopus laevis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Xenopus laevis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Xenopus laevis
General Information
General Information
Commentary
Organism
evolution
the CD38-cADPR signaling system is conserved during vertebrate evolution, phylogenetic tree
Xenopus laevis
physiological function
the enzyme is CD38-cADPR signaling system in frog cells
Xenopus laevis
General Information (protein specific)
General Information
Commentary
Organism
evolution
the CD38-cADPR signaling system is conserved during vertebrate evolution, phylogenetic tree
Xenopus laevis
physiological function
the enzyme is CD38-cADPR signaling system in frog cells
Xenopus laevis
Other publictions for EC 3.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749830
Chini
The NADase CD38 is induced by ...
Homo sapiens
Biochem. Biophys. Res. Commun.
513
486-493
2019
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1
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1
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1
1
1
1
-
-
750282
Shu
Blockade of CD38 diminishes l ...
Mus musculus
Cell. Signal.
42
249-258
2018
-
-
-
-
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4
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1
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1
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1
1
1
1
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731357
Kuhn
Probing the catalytic mechanis ...
Bos taurus
Biochim. Biophys. Acta
1844
1317-1331
2014
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-
-
-
11
-
7
12
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-
1
-
2
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1
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3
-
1
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12
1
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11
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7
-
12
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-
1
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-
3
-
1
-
-
12
1
-
-
-
-
1
1
-
12
12
731882
Ma
Basal CD38/cyclic ADP-ribose-d ...
Mus musculus
Glia
62
943-955
2014
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-
-
-
-
-
-
-
-
-
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4
-
1
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4
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4
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1
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1
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4
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4
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4
-
1
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-
1
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-
-
-
2
2
-
-
-
731987
Shrimp
Revealing CD38 cellular locali ...
Homo sapiens
J. Am. Chem. Soc.
136
5656-5663
2014
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-
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2
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2
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3
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1
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2
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3
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1
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1
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2
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2
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3
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2
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3
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1
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1
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1
1
1
1
-
-
731119
Jiang
Identification of ADP-ribosyla ...
Homo sapiens
Anal. Biochem.
433
218-226
2013
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6
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1
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1
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1
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1
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1
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1
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1
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6
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1
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1
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1
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1
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1
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1
1
-
-
-
732593
Coskun
Purification of NAD+ glycohydr ...
Homo sapiens
Oncol. Lett.
6
227-231
2013
-
-
-
-
-
-
-
-
2
-
2
3
-
1
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1
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1
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3
1
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1
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2
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2
3
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1
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1
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3
1
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1
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731296
Kwong
Catalysis-based inhibitors of ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochemistry
51
555-564
2012
-
-
-
1
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-
42
-
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9
-
3
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4
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9
-
3
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3
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1
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42
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9
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4
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9
-
3
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3
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9
9
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732501
Ikeda
Identification of a major enzy ...
Xenopus laevis
Mol. Cell. Biochem.
366
69-80
2012
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1
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3
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2
4
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3
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1
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3
1
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4
1
1
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1
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1
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3
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2
4
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1
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3
1
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4
1
1
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1
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2
2
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-
732691
Egea
Insights into the mechanism of ...
Bos taurus
PLoS ONE
7
e34918
2012
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-
1
1
6
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7
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2
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2
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1
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1
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2
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1
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1
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1
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6
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7
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2
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1
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2
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1
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1
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1
1
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714434
Kellenberger
Flavonoids as inhibitors of hu ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
3939-3942
2011
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1
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17
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1
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1
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2
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16
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1
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16
17
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2
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714921
Congleton
ATRA-induced HL-60 myeloid leu ...
Homo sapiens
Exp. Cell Res.
317
910-919
2011
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1
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1
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1
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1
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1
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1
1
1
1
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716910
Lee
Cyclic ADP-ribose and NAADP: f ...
Mus musculus
Sci. China Life Sci.
54
699-711
2011
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1
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3
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3
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1
1
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326409
Orsomando
NAD(P)+-glycohydrolase from hu ...
Homo sapiens
Comp. Biochem. Physiol. B
126
89-98
2000
-
-
-
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-
2
7
3
2
3
2
1
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3
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1
1
1
1
3
3
22
1
1
1
1
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1
1
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1
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2
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7
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3
2
3
2
1
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1
1
1
3
3
22
1
1
1
1
-
1
1
-
1
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326410
Berthelier
-
Human CD38 is an authentic NAD ...
Homo sapiens
Biochem. J.
330
1383-1390
1998
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6
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9
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9
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326411
Chini
Enzymatic synthesis and degrad ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
209
167-174
1995
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326406
Jorge
Carbon source regulation of ni ...
Neurospora crassa
J. Gen. Microbiol.
130
1563-1568
1984
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326403
Yamasaki
-
Purification and some properti ...
Saccharomyces cerevisiae
J. Ferment. Technol.
60
131-137
1982
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2
1
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2
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1
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2
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1
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2
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1
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2
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-
326407
Skala
Elevated NAD(P) glycohydrolase ...
Homo sapiens
Blood
60
912-917
1982
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-
-
-
-
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1
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1
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1
-
-
-
-
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326408
Artman
Nicotinamide adenine dinucleot ...
Oryctolagus cuniculus, Ovis aries
Can. J. Microbiol.
28
696-702
1982
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-
-
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2
-
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-
-
2
-
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1
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2
-
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1
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326405
Broeker
-
A nicotinamide adenine dinucle ...
Streptomyces griseus
FEMS Microbiol. Lett.
6
245-247
1979
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-
-
-
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1
2
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1
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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1
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326404
Mather
A heat-stable nicotinamide-ade ...
Pseudomonas putida KB1
Biochem. J.
129
141-152
1972
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1
5
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1
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1
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1
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2
5
-
1
-
1
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2
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2
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1
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5
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1
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1
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2
5
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1
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1
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2
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2
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