BRENDA - Enzyme Database
show all sequences of 3.2.2.6

Basal CD38/cyclic ADP-ribose-dependent signaling mediates ATP release and survival of microglia by modulating connexin 43 hemichannels

Ma, Y.; Cao, W.; Wang, L.; Jiang, J.; Nie, H.; Wang, B.; Wei, X.; Ying, W.; Glia 62, 943-955 (2014)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclic ADP-ribose + H2O
Mus musculus
-
ADP-D-ribose
-
-
?
additional information
Mus musculus
CD38 is a NAD+-dependent, multifunctional ectoenzyme that cannot only generate cyclic ADP-ribose from NAD+ but also hydrolyze cyclic ADP-ribose to ADP-ribose and transport cyclic ADP-ribose into cells
?
-
-
-
NAD+
Mus musculus
-
cyclic ADP-ribose + nicotinamide
-
-
?
NAD+ + H2O
Mus musculus
overall reaction
ADP-D-ribose + nicotinamide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
P56528
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
astrocyte
-
Mus musculus
-
brain
-
Mus musculus
-
BV-2 cell
-
Mus musculus
-
microglia
primary microglial cultures as cellular models
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclic ADP-ribose + H2O
-
731882
Mus musculus
ADP-D-ribose
-
-
-
?
additional information
CD38 is a NAD+-dependent, multifunctional ectoenzyme that cannot only generate cyclic ADP-ribose from NAD+ but also hydrolyze cyclic ADP-ribose to ADP-ribose and transport cyclic ADP-ribose into cells
731882
Mus musculus
?
-
-
-
-
NAD+
-
731882
Mus musculus
cyclic ADP-ribose + nicotinamide
-
-
-
?
NAD+ + H2O
overall reaction
731882
Mus musculus
ADP-D-ribose + nicotinamide
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclic ADP-ribose + H2O
Mus musculus
-
ADP-D-ribose
-
-
?
additional information
Mus musculus
CD38 is a NAD+-dependent, multifunctional ectoenzyme that cannot only generate cyclic ADP-ribose from NAD+ but also hydrolyze cyclic ADP-ribose to ADP-ribose and transport cyclic ADP-ribose into cells
?
-
-
-
NAD+
Mus musculus
-
cyclic ADP-ribose + nicotinamide
-
-
?
NAD+ + H2O
Mus musculus
overall reaction
ADP-D-ribose + nicotinamide
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
astrocyte
-
Mus musculus
-
brain
-
Mus musculus
-
BV-2 cell
-
Mus musculus
-
microglia
primary microglial cultures as cellular models
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyclic ADP-ribose + H2O
-
731882
Mus musculus
ADP-D-ribose
-
-
-
?
additional information
CD38 is a NAD+-dependent, multifunctional ectoenzyme that cannot only generate cyclic ADP-ribose from NAD+ but also hydrolyze cyclic ADP-ribose to ADP-ribose and transport cyclic ADP-ribose into cells
731882
Mus musculus
?
-
-
-
-
NAD+
-
731882
Mus musculus
cyclic ADP-ribose + nicotinamide
-
-
-
?
NAD+ + H2O
overall reaction
731882
Mus musculus
ADP-D-ribose + nicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
General Information
General Information
Commentary
Organism
malfunction
CD38 reductions lead to microglial apoptosis. inhibition of CD38/cADPR-dependent signaling by CD38 silencing or 8-bromo-cADPR, a ryanodine receptor antagonist, produced significant ATP release from BV2 microglia. Cx43 small interfering RNA and Cx43 hemichannel blocker 18-alpha-glycyrrhetinic acid completely prevented the CD38 silencing or 8-bromo-cADPR-induced ATP release. Prevention of the ATP release might also be due to P2X7 receptor antagonists. Key role of ATP release in the microglial apoptosis induced by decreased CD38/cADPR-dependent signaling, overview
Mus musculus
physiological function
CD38 is an ectoenzyme that consumes NAD+ to produce cyclic ADP-ribose, a potent agonist of ryanodine receptors. Basal CD38/cyclic ADP-ribose-dependent signaling plays a key role in ATP release, which mediates basal survival of microglia, overview
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
CD38 reductions lead to microglial apoptosis. inhibition of CD38/cADPR-dependent signaling by CD38 silencing or 8-bromo-cADPR, a ryanodine receptor antagonist, produced significant ATP release from BV2 microglia. Cx43 small interfering RNA and Cx43 hemichannel blocker 18-alpha-glycyrrhetinic acid completely prevented the CD38 silencing or 8-bromo-cADPR-induced ATP release. Prevention of the ATP release might also be due to P2X7 receptor antagonists. Key role of ATP release in the microglial apoptosis induced by decreased CD38/cADPR-dependent signaling, overview
Mus musculus
physiological function
CD38 is an ectoenzyme that consumes NAD+ to produce cyclic ADP-ribose, a potent agonist of ryanodine receptors. Basal CD38/cyclic ADP-ribose-dependent signaling plays a key role in ATP release, which mediates basal survival of microglia, overview
Mus musculus
Other publictions for EC 3.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749830
Chini
The NADase CD38 is induced by ...
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Biochem. Biophys. Res. Commun.
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2019
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1
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750282
Shu
Blockade of CD38 diminishes l ...
Mus musculus
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731357
Kuhn
Probing the catalytic mechanis ...
Bos taurus
Biochim. Biophys. Acta
1844
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2014
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11
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7
12
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1
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12
1
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1
1
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12
12
731882
Ma
Basal CD38/cyclic ADP-ribose-d ...
Mus musculus
Glia
62
943-955
2014
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Shrimp
Revealing CD38 cellular locali ...
Homo sapiens
J. Am. Chem. Soc.
136
5656-5663
2014
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2
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731119
Jiang
Identification of ADP-ribosyla ...
Homo sapiens
Anal. Biochem.
433
218-226
2013
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732593
Coskun
Purification of NAD+ glycohydr ...
Homo sapiens
Oncol. Lett.
6
227-231
2013
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731296
Kwong
Catalysis-based inhibitors of ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochemistry
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2012
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1
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9
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Ikeda
Identification of a major enzy ...
Xenopus laevis
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2
2
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732691
Egea
Insights into the mechanism of ...
Bos taurus
PLoS ONE
7
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1
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6
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7
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Kellenberger
Flavonoids as inhibitors of hu ...
Homo sapiens
Bioorg. Med. Chem. Lett.
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17
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Congleton
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Homo sapiens
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Rattus norvegicus
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326406
Jorge
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Neurospora crassa
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Yamasaki
-
Purification and some properti ...
Saccharomyces cerevisiae
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326407
Skala
Elevated NAD(P) glycohydrolase ...
Homo sapiens
Blood
60
912-917
1982
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326408
Artman
Nicotinamide adenine dinucleot ...
Oryctolagus cuniculus, Ovis aries
Can. J. Microbiol.
28
696-702
1982
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326405
Broeker
-
A nicotinamide adenine dinucle ...
Streptomyces griseus
FEMS Microbiol. Lett.
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245-247
1979
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326404
Mather
A heat-stable nicotinamide-ade ...
Pseudomonas putida KB1
Biochem. J.
129
141-152
1972
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