BRENDA - Enzyme Database
show all sequences of 3.2.2.6

Identification of ADP-ribosylation sites of CD38 mutants by precursor ion scanning mass spectrometry

Jiang, H.; Sherwood, R.; Zhang, S.; Zhu, X.; Liu, Q.; Graeff, R.; Kriksunov, I.A.; Lee, H.C.; Hao, Q.; Lin, H.; Anal. Biochem. 433, 218-226 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E226D
site-directed mutagenesis, catalytic mutant
Homo sapiens
E226Q
site-directed mutagenesis, catalytic mutant
Homo sapiens
N100D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N164D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N209D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N219D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + H2O
Homo sapiens
via a stepwise reaction mechanism
ADP-D-ribose + nicotinamide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P28907
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
N-glycosylation sites are N100, N164, N209, and N219
Homo sapiens
Reaction
Reaction
Commentary
Organism
NAD+ + H2O = ADP-D-ribose + nicotinamide
reaction mechanism, overview
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + H2O
via a stepwise reaction mechanism
731119
Homo sapiens
ADP-D-ribose + nicotinamide
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E226D
site-directed mutagenesis, catalytic mutant
Homo sapiens
E226Q
site-directed mutagenesis, catalytic mutant
Homo sapiens
N100D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N164D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N209D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
N219D
site-directed mutagenesis, N-glycoylation at the site is abolished
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + H2O
Homo sapiens
via a stepwise reaction mechanism
ADP-D-ribose + nicotinamide
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
N-glycosylation sites are N100, N164, N209, and N219
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + H2O
via a stepwise reaction mechanism
731119
Homo sapiens
ADP-D-ribose + nicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
additional information
D226/Q226 and K129 residues of the two CD38 enzyme are the ADP-ribosylation sites. 6-Alkyne-F-araNAD, 6-alkyne-NAD, and Rh-N3 are used in the labeling reactions of CD38 wild-type and mutants, overview
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
additional information
D226/Q226 and K129 residues of the two CD38 enzyme are the ADP-ribosylation sites. 6-Alkyne-F-araNAD, 6-alkyne-NAD, and Rh-N3 are used in the labeling reactions of CD38 wild-type and mutants, overview
Homo sapiens
Other publictions for EC 3.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749830
Chini
The NADase CD38 is induced by ...
Homo sapiens
Biochem. Biophys. Res. Commun.
513
486-493
2019
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
750282
Shu
Blockade of CD38 diminishes l ...
Mus musculus
Cell. Signal.
42
249-258
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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1
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1
-
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-
-
-
-
-
-
-
1
1
1
1
-
-
731357
Kuhn
Probing the catalytic mechanis ...
Bos taurus
Biochim. Biophys. Acta
1844
1317-1331
2014
-
-
-
-
11
-
7
12
-
-
-
1
-
2
-
-
-
1
-
-
-
-
3
-
1
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
11
-
-
7
-
12
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
12
1
-
-
-
-
1
1
-
12
12
731882
Ma
Basal CD38/cyclic ADP-ribose-d ...
Mus musculus
Glia
62
943-955
2014
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
4
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
4
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
731987
Shrimp
Revealing CD38 cellular locali ...
Homo sapiens
J. Am. Chem. Soc.
136
5656-5663
2014
-
-
-
-
2
-
-
-
2
-
-
3
-
1
-
-
-
-
-
2
-
-
3
-
1
-
-
-
1
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-
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-
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-
-
2
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-
-
2
-
-
3
-
-
-
-
-
2
-
-
3
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
731119
Jiang
Identification of ADP-ribosyla ...
Homo sapiens
Anal. Biochem.
433
218-226
2013
-
-
-
-
6
-
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
732593
Coskun
Purification of NAD+ glycohydr ...
Homo sapiens
Oncol. Lett.
6
227-231
2013
-
-
-
-
-
-
-
-
2
-
2
3
-
1
-
-
1
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
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-
2
-
2
3
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
731296
Kwong
Catalysis-based inhibitors of ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochemistry
51
555-564
2012
-
-
-
1
-
-
42
-
-
-
-
9
-
3
-
-
-
-
-
4
-
-
9
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
42
-
-
-
-
-
9
-
-
-
-
-
4
-
-
9
-
3
-
-
-
3
-
-
-
-
9
9
-
-
-
732501
Ikeda
Identification of a major enzy ...
Xenopus laevis
Mol. Cell. Biochem.
366
69-80
2012
-
-
1
-
-
-
-
-
3
-
2
4
-
3
-
1
-
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3
1
-
4
1
1
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1
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1
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3
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2
4
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1
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3
1
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732691
Egea
Insights into the mechanism of ...
Bos taurus
PLoS ONE
7
e34918
2012
-
-
1
1
6
-
-
7
-
-
-
2
-
2
-
1
-
1
-
-
-
-
2
-
1
-
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1
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1
-
1
6
-
-
-
-
7
-
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2
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1
-
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-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
714434
Kellenberger
Flavonoids as inhibitors of hu ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
3939-3942
2011
-
-
1
-
-
-
17
-
-
-
-
-
-
1
-
-
1
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-
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2
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16
-
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1
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16
17
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1
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-
2
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-
-
-
-
-
-
-
-
-
-
-
-
714921
Congleton
ATRA-induced HL-60 myeloid leu ...
Homo sapiens
Exp. Cell Res.
317
910-919
2011
-
-
-
-
1
-
1
-
1
-
-
-
-
1
-
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-
1
-
-
1
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1
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716910
Lee
Cyclic ADP-ribose and NAADP: f ...
Mus musculus
Sci. China Life Sci.
54
699-711
2011
-
-
-
-
-
-
-
-
1
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3
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1
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1
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1
-
-
3
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
326409
Orsomando
NAD(P)+-glycohydrolase from hu ...
Homo sapiens
Comp. Biochem. Physiol. B
126
89-98
2000
-
-
-
-
-
2
7
3
2
3
2
1
-
3
-
-
1
1
1
1
3
3
22
1
1
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
2
-
7
-
3
2
3
2
1
-
-
-
1
1
1
3
3
22
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
326410
Berthelier
-
Human CD38 is an authentic NAD ...
Homo sapiens
Biochem. J.
330
1383-1390
1998
-
-
-
-
-
-
1
6
-
-
-
-
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1
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1
-
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9
-
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1
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6
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1
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9
-
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-
-
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-
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-
326411
Chini
Enzymatic synthesis and degrad ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
209
167-174
1995
-
-
-
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-
-
-
-
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2
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4
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1
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4
-
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1
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-
326406
Jorge
Carbon source regulation of ni ...
Neurospora crassa
J. Gen. Microbiol.
130
1563-1568
1984
-
-
-
-
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-
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1
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3
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2
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-
326403
Yamasaki
-
Purification and some properti ...
Saccharomyces cerevisiae
J. Ferment. Technol.
60
131-137
1982
-
-
-
-
-
-
2
1
-
-
2
-
-
1
-
-
1
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2
-
2
-
1
-
2
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1
-
1
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2
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1
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2
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1
-
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2
-
2
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
326407
Skala
Elevated NAD(P) glycohydrolase ...
Homo sapiens
Blood
60
912-917
1982
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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-
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-
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-
326408
Artman
Nicotinamide adenine dinucleot ...
Oryctolagus cuniculus, Ovis aries
Can. J. Microbiol.
28
696-702
1982
-
-
-
-
-
-
-
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2
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2
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1
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2
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1
-
-
-
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-
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-
-
-
-
-
-
-
-
326405
Broeker
-
A nicotinamide adenine dinucle ...
Streptomyces griseus
FEMS Microbiol. Lett.
6
245-247
1979
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
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-
-
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1
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1
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1
-
1
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1
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2
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1
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1
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1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
326404
Mather
A heat-stable nicotinamide-ade ...
Pseudomonas putida KB1
Biochem. J.
129
141-152
1972
-
-
-
-
-
-
1
5
-
-
1
-
-
1
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1
-
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-
-
2
5
-
1
-
1
-
2
-
2
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-
-
-
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-
1
-
5
-
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1
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1
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2
5
-
1
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1
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2
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2
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