BRENDA - Enzyme Database
show all sequences of 3.2.2.6

ATRA-induced HL-60 myeloid leukemia cell differentiation depends on the CD38 cytosolic tail needed for membrane localization, but CD38 enzymatic activity is unnecessary

Congleton, J.; Jiang, H.; Malavasi, F.; Lin, H.; Yen, A.; Exp. Cell Res. 317, 910-919 (2011)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E226Q
the mutation cripples enzymatic activity
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
arabinosyl 2'-fluoro-2'-deoxy-NAD
suicide substrate that inhibits CD38 ectoenzyme activity
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
CD38 is a type II membrane protein with an extracellular C-terminal enzymatic domain with NADase/NADPase and ADPR cyclase activity and a short cytoplasmic N-terminal tail
Homo sapiens
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HL-60 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NGD+ + H2O
-
714921
Homo sapiens
cyclic GDP-ribose
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E226Q
the mutation cripples enzymatic activity
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arabinosyl 2'-fluoro-2'-deoxy-NAD
suicide substrate that inhibits CD38 ectoenzyme activity
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
CD38 is a type II membrane protein with an extracellular C-terminal enzymatic domain with NADase/NADPase and ADPR cyclase activity and a short cytoplasmic N-terminal tail
Homo sapiens
16020
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HL-60 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NGD+ + H2O
-
714921
Homo sapiens
cyclic GDP-ribose
-
-
-
?
Expression
Organism
Commentary
Expression
Homo sapiens
0.001 mM ATRA induces CD38 expression
up
General Information
General Information
Commentary
Organism
physiological function
leukocyte antigen CD38 expression is an early marker of all-trans retinoic acid-stimulated differentiation in the leukemic cell line HL-60 where CD38 promotes induced myeloid maturation when overexpressed. The ability of CD38 to propel all-trans retinoic acid-induced myeloid differentiation and G1/0 arrest is unimpaired by loss of its ectoenzymeactivity
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
leukocyte antigen CD38 expression is an early marker of all-trans retinoic acid-stimulated differentiation in the leukemic cell line HL-60 where CD38 promotes induced myeloid maturation when overexpressed. The ability of CD38 to propel all-trans retinoic acid-induced myeloid differentiation and G1/0 arrest is unimpaired by loss of its ectoenzymeactivity
Homo sapiens
Expression (protein specific)
Organism
Commentary
Expression
Homo sapiens
0.001 mM ATRA induces CD38 expression
up
Other publictions for EC 3.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749830
Chini
The NADase CD38 is induced by ...
Homo sapiens
Biochem. Biophys. Res. Commun.
513
486-493
2019
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1
1
1
1
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-
750282
Shu
Blockade of CD38 diminishes l ...
Mus musculus
Cell. Signal.
42
249-258
2018
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1
1
1
1
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731357
Kuhn
Probing the catalytic mechanis ...
Bos taurus
Biochim. Biophys. Acta
1844
1317-1331
2014
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11
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7
12
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12
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3
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1
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12
1
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1
1
-
12
12
731882
Ma
Basal CD38/cyclic ADP-ribose-d ...
Mus musculus
Glia
62
943-955
2014
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4
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1
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4
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4
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1
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1
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2
2
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731987
Shrimp
Revealing CD38 cellular locali ...
Homo sapiens
J. Am. Chem. Soc.
136
5656-5663
2014
-
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2
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2
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1
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2
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3
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1
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1
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1
1
1
1
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731119
Jiang
Identification of ADP-ribosyla ...
Homo sapiens
Anal. Biochem.
433
218-226
2013
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6
-
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1
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732593
Coskun
Purification of NAD+ glycohydr ...
Homo sapiens
Oncol. Lett.
6
227-231
2013
-
-
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2
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2
3
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1
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731296
Kwong
Catalysis-based inhibitors of ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochemistry
51
555-564
2012
-
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1
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42
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9
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3
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4
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9
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3
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3
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1
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42
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9
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4
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9
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3
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3
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9
9
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732501
Ikeda
Identification of a major enzy ...
Xenopus laevis
Mol. Cell. Biochem.
366
69-80
2012
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1
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3
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4
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1
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4
1
1
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1
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2
2
-
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-
732691
Egea
Insights into the mechanism of ...
Bos taurus
PLoS ONE
7
e34918
2012
-
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1
1
6
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7
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2
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2
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1
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6
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714434
Kellenberger
Flavonoids as inhibitors of hu ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
3939-3942
2011
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1
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17
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17
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714921
Congleton
ATRA-induced HL-60 myeloid leu ...
Homo sapiens
Exp. Cell Res.
317
910-919
2011
-
-
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1
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1
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1
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1
1
1
1
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716910
Lee
Cyclic ADP-ribose and NAADP: f ...
Mus musculus
Sci. China Life Sci.
54
699-711
2011
-
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1
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3
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1
1
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326409
Orsomando
NAD(P)+-glycohydrolase from hu ...
Homo sapiens
Comp. Biochem. Physiol. B
126
89-98
2000
-
-
-
-
-
2
7
3
2
3
2
1
-
3
-
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1
1
1
1
3
3
22
1
1
1
1
-
1
1
-
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-
1
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-
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-
-
-
-
2
-
7
-
3
2
3
2
1
-
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1
1
1
3
3
22
1
1
1
1
-
1
1
-
1
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326410
Berthelier
-
Human CD38 is an authentic NAD ...
Homo sapiens
Biochem. J.
330
1383-1390
1998
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1
6
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9
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6
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1
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9
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326411
Chini
Enzymatic synthesis and degrad ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
209
167-174
1995
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4
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1
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326406
Jorge
Carbon source regulation of ni ...
Neurospora crassa
J. Gen. Microbiol.
130
1563-1568
1984
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326403
Yamasaki
-
Purification and some properti ...
Saccharomyces cerevisiae
J. Ferment. Technol.
60
131-137
1982
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-
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2
1
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2
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1
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2
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1
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2
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2
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1
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2
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1
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1
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326407
Skala
Elevated NAD(P) glycohydrolase ...
Homo sapiens
Blood
60
912-917
1982
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326408
Artman
Nicotinamide adenine dinucleot ...
Oryctolagus cuniculus, Ovis aries
Can. J. Microbiol.
28
696-702
1982
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326405
Broeker
-
A nicotinamide adenine dinucle ...
Streptomyces griseus
FEMS Microbiol. Lett.
6
245-247
1979
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1
2
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326404
Mather
A heat-stable nicotinamide-ade ...
Pseudomonas putida KB1
Biochem. J.
129
141-152
1972
-
-
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1
5
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1
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1
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1
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2
5
-
1
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1
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2
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2
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1
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5
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1
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1
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2
5
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1
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1
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2
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