General Stability | Organism |
---|---|
50% inactivation at 2 M urea | Homo sapiens |
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity | Homo sapiens | |
Cr3+ | inhibition of NADase and adenosine diphosphate cyclase | Homo sapiens | |
Hg2+ | complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase | Homo sapiens | |
Pb2+ | complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase | Homo sapiens | |
SDS | - |
Homo sapiens | |
Sn2+ | complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase | Homo sapiens | |
Zn2+ | inhibition of NADase and adenosine diphosphate cyclase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NAD+ | - |
Homo sapiens | |
0.026 | - |
NAD+ | pH 7.0, 28°C | Homo sapiens | |
0.033 | - |
epsilonNAD+ | - |
Homo sapiens | |
0.033 | - |
nicotinamide 1,N6-ethenoadenine dinucleotide | pH 7.0, 28°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase | Homo sapiens | |
Ag+ | at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity | Homo sapiens | |
Cu2+ | stimulation of cADPR activity | Homo sapiens | |
additional information | no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column | Homo sapiens |
45000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Homo sapiens |
partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ | also catalyses transfer of ADP-ribose(P) residues | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
partially purified enzyme, ADPR activity | Homo sapiens |
0.113 | - |
partially purified enzyme, cADPR activity | Homo sapiens |
2.205 | - |
partially purified enzyme, NADase activity | Homo sapiens |
Storage Stability | Organism |
---|---|
20°C, lyophilized enzyme, 90% remaining activity after 6 months | Homo sapiens |
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable | Homo sapiens |
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetylpyridine + adenosine diphosphoribose | transglycosylation activity, very poor hydrolytic activity with this substrate | Homo sapiens | 3-acetylpyridine adenine dinucleotide + phosphate | - |
? | |
3-acetylpyridine adenine dinucleotide + H2O | - |
Homo sapiens | ? | - |
? | |
3-acetylpyridine hypoxanthine dinucleotide + H2O | low activity | Homo sapiens | ? | - |
? | |
3-pyridine adenine dinucleotide + H2O | - |
Homo sapiens | ? | - |
? | |
3-pyridinealdehyde hypoxanthine dinucleotide + H2O | - |
Homo sapiens | ? | - |
? | |
3-pyridinealdehyde hypoxanthine dinucleotide + H2O | 60% activity compared to the activity with NAD+ | Homo sapiens | ? | - |
? | |
cyclic ADP-ribose + H2O | - |
Homo sapiens | ? | - |
? | |
cyclic ADP-ribose + H2O | i.e. ADPR activity | Homo sapiens | adenosine diphosphoribose + ? | - |
? | |
epsilon-NAD+ + H2O | - |
Homo sapiens | ? | - |
? | |
epsilon-NADP+ + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose | Homo sapiens | ? | - |
? | |
additional information | enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose | Homo sapiens | ? | - |
? | |
NAD+ | - |
Homo sapiens | cyclic ADP-ribose + nicotinamide | - |
? | |
NAD+ + ? | i.e. cADPR activity | Homo sapiens | cyclic ADP-ribose + ? | - |
? | |
NAD+ + H2O | - |
Homo sapiens | ADP-ribose + nicotinamide | - |
? | |
NADP+ + H2O | - |
Homo sapiens | nicotinamide + ADPribose-phosphate | - |
? | |
NADP+ + H2O | 80% activity compared to the activity with NAD+ | Homo sapiens | ADP-ribose-P + nicotinamide | - |
? | |
NGD+ + H2O | as effective as substrate as NAD+ | Homo sapiens | GDP-ribose + nicotinamide | - |
? | |
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O | i.e. epsilonNAD+, used for a fluorometric assay | Homo sapiens | ? | - |
? | |
nicotinamide guanine dinucleotide + H2O | - |
Homo sapiens | ? | - |
? | |
nicotinamide-hypoxanthine dinucleotide + H2O | - |
Homo sapiens | ? | - |
? | |
thio-NAD+ + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 45000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)+-glycohydrolase | - |
Homo sapiens |
NADase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 50 | inactive above 50°C | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
half-life: about 1 day | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |
7 | - |
NADase activity | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | - |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isoelectric focusing | - |
4.9 |