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Literature summary for 3.2.2.6 extracted from

  • Orsomando, G.; Polzonetti, V.; Natalini, P.
    NAD(P)+-glycohydrolase from human spleen: a multicatalytic enzyme (2000), Comp. Biochem. Physiol. B, 126, 89-98.
    View publication on PubMed

General Stability

General Stability Organism
50% inactivation at 2 M urea Homo sapiens
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity Homo sapiens
Cr3+ inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
Hg2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
Pb2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
SDS
-
Homo sapiens
Sn2+ complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase Homo sapiens
Zn2+ inhibition of NADase and adenosine diphosphate cyclase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
NAD+
-
Homo sapiens
0.026
-
NAD+ pH 7.0, 28°C Homo sapiens
0.033
-
epsilonNAD+
-
Homo sapiens
0.033
-
nicotinamide 1,N6-ethenoadenine dinucleotide pH 7.0, 28°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase Homo sapiens
Ag+ at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity Homo sapiens
Cu2+ stimulation of cADPR activity Homo sapiens
additional information no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column Homo sapiens
45000
-
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens
partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ also catalyses transfer of ADP-ribose(P) residues Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
partially purified enzyme, ADPR activity Homo sapiens
0.113
-
partially purified enzyme, cADPR activity Homo sapiens
2.205
-
partially purified enzyme, NADase activity Homo sapiens

Storage Stability

Storage Stability Organism
20°C, lyophilized enzyme, 90% remaining activity after 6 months Homo sapiens
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable Homo sapiens
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetylpyridine + adenosine diphosphoribose transglycosylation activity, very poor hydrolytic activity with this substrate Homo sapiens 3-acetylpyridine adenine dinucleotide + phosphate
-
?
3-acetylpyridine adenine dinucleotide + H2O
-
Homo sapiens ?
-
?
3-acetylpyridine hypoxanthine dinucleotide + H2O low activity Homo sapiens ?
-
?
3-pyridine adenine dinucleotide + H2O
-
Homo sapiens ?
-
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
-
Homo sapiens ?
-
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O 60% activity compared to the activity with NAD+ Homo sapiens ?
-
?
cyclic ADP-ribose + H2O
-
Homo sapiens ?
-
?
cyclic ADP-ribose + H2O i.e. ADPR activity Homo sapiens adenosine diphosphoribose + ?
-
?
epsilon-NAD+ + H2O
-
Homo sapiens ?
-
?
epsilon-NADP+ + H2O
-
Homo sapiens ?
-
?
additional information enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose Homo sapiens ?
-
?
additional information enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose Homo sapiens ?
-
?
NAD+
-
Homo sapiens cyclic ADP-ribose + nicotinamide
-
?
NAD+ + ? i.e. cADPR activity Homo sapiens cyclic ADP-ribose + ?
-
?
NAD+ + H2O
-
Homo sapiens ADP-ribose + nicotinamide
-
?
NADP+ + H2O
-
Homo sapiens nicotinamide + ADPribose-phosphate
-
?
NADP+ + H2O 80% activity compared to the activity with NAD+ Homo sapiens ADP-ribose-P + nicotinamide
-
?
NGD+ + H2O as effective as substrate as NAD+ Homo sapiens GDP-ribose + nicotinamide
-
?
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O i.e. epsilonNAD+, used for a fluorometric assay Homo sapiens ?
-
?
nicotinamide guanine dinucleotide + H2O
-
Homo sapiens ?
-
?
nicotinamide-hypoxanthine dinucleotide + H2O
-
Homo sapiens ?
-
?
thio-NAD+ + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NAD(P)+-glycohydrolase
-
Homo sapiens
NADase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
38 50 inactive above 50°C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38
-
half-life: about 1 day Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens
7
-
NADase activity Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
4.9