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Literature summary for 3.2.2.5 extracted from
- NAD-dependent inhibition of the NAD-glycohydrolase activity in A549 cells (2002), Mol. Cell. Biochem., 233, 127-132.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-NAD+ | substrate inhibition, mediated by ADP-ribosylation, partially reversible by arginine or histidine, inhibits apoptosis in vivo | Homo sapiens |  |
| beta-NADP+ | in vivo | Homo sapiens | |
| nicotinic acid | slight inhibition, in vivo | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
NAD+ | membrane fraction, pH 7.0, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | outer cell surface, GPI-anchored | Homo sapiens | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + H2O | Homo sapiens | - |
ADP-ribose + nicotinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, solubilization from the membrane fraction, best with CHAPS or Triton X-100 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-549 cell | lung epithelial cell line | Homo sapiens | - |
| epithelium | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,N6-etheno-NAD+ + H2O | - |
Homo sapiens | ? | - |
? | |
| alpha-NAD+ + H2O | - |
Homo sapiens | ADP-ribose + nicotinamide | - |
? | |
| beta-NAD+ + H2O | - |
Homo sapiens | ADP-ribose + nicotinamide | - |
? | |
| NAD+ + H2O | - |
Homo sapiens | ADP-ribose + nicotinamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD-glycohydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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