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Literature summary for 3.2.2.5 extracted from
- Vibrio fischeri genes hvnA and hvnB encode secreted NAD(+)-glycohydrolases (2001), J. Bacteriol., 183, 309-317.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene hvnA, part of a four gene operon, and monocistronic gene hvnB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha and BW23474 | Aliivibrio fischeri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of inactive hvnA/hvnB null mutant | Aliivibrio fischeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | inhibits the reaction between ADP-ribose and polypeptides | Aliivibrio fischeri |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Aliivibrio fischeri | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, recombinant enzyme HvnB, SDS-PAGE | Aliivibrio fischeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aliivibrio fischeri | Q56685 | isolated from Euprymna scolopes, genes hvnA and hvnB | - |
| Aliivibrio fischeri | Q9F0R5 | isolated from Euprymna scolopes light organ, genes hvnA and hvnB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzymes partially from culture supernatant, recombinant enzymes from Escherichia coli | Aliivibrio fischeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,N6-etheno-NAD+ + H2O | - |
Aliivibrio fischeri | ? | - |
? | |
| NAD+ + H2O | - |
Aliivibrio fischeri | ADP-ribose + nicotinamide | ADP-ribose reacts further with polyarginine, polyhistidine, or polylysine | ? | |
| NAD+ + H2O | - |
Aliivibrio fischeri | ADP-ribose + nicotinamide | ADP-ribose reacts spontaneously with polyarginine, polyhistidine, or polylysine | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, recombinant enzyme HvnB, SDS-PAGE | Aliivibrio fischeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HvnA | - |
Aliivibrio fischeri |
| HvnB | - |
Aliivibrio fischeri |
| NAD+-glycohydrolase | - |
Aliivibrio fischeri |
| NADase | - |
Aliivibrio fischeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aliivibrio fischeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aliivibrio fischeri |
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Release 2023.1 (January 2023)
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