Literature summary for 3.2.2.5 extracted from

Muller-Steffner, H.; Schenherr-Gusse, I.; Tarnus, C.; Schuber, F.
Calf spleen NAD+ glycohydrolase: solubilization, purification, and properties of the intact form of the enzyme (1993), Arch. Biochem. Biophys., 304, 154-162.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	NAD+	-	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NAD+ + H2O	Bos taurus	-	ADPribose + nicotinamide	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	calf	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
180	-	-	Bos taurus

Storage Stability

Storage Stability	Organism
-20°C, emulphogene, several months	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + H2O	-	Bos taurus	ADPribose + nicotinamide	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	24 h in 0.1%, w/v emulphogene 85% of activity is remained	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
53.6	-	NAD+	-	Bos taurus

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Release 2023.1 (January 2023)