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Literature summary for 3.2.2.5 extracted from

  • Jorcke, D.; Ziegler, M.; Schweiger, M.
    Characteriation of hydrosoluble and detergent-solubilized forms of mitochondrial NAD+ glycohydrolase from bovine liver (1997), Adv. Exp. Med. Biol., 419, 447-451.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
EDTA detergent solubilized NADase, not the steapsin treated enzyme Bos taurus
NaCl
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 epsilon-NAD+ steapsin treated enzyme Bos taurus
0.01
-
 epsilon-NAD+ detergent solubilized NADase Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ bivalent cations for example Zn2+, not Mg2+ Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Bos taurus
-
 ADPribose + nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epsilon-NAD+ + H2O
-
 Bos taurus ADP-D-ribose + nicotinamide
-
 ?
NAD+ + H2O
-
 Bos taurus ADPribose + nicotinamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
 steapsin treated enzyme Bos taurus
39
-
 detergent solubilized NADase Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 detergent solubilized NADase Bos taurus
8.5
-
 steapsin treated enzyme Bos taurus