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Literature summary for 3.2.2.30 extracted from

  • Wang, S.; Thomas, K.; Schramm, V.L.
    Catalytic site cooperativity in dimeric methylthioadenosine nucleosidase (2014), Biochemistry, 53, 1527-1535.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
E173Q inactive Staphylococcus aureus
F104C/C186S the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
methylthio-DADMe-immucillin-A i.e. (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-amino-6-deoxyfutalosine + H2O Staphylococcus aureus
-
 dehypoxanthine futalosine + adenine
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis-2-nitrobenzoic acid + H2O
-
 Staphylococcus aureus ?
-
 ?
6-amino-6-deoxyfutalosine + H2O
-
 Staphylococcus aureus dehypoxanthine futalosine + adenine
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Staphylococcus aureus

Synonyms

Synonyms Comment Organism
methylthioadenosine nucleosidase
-
 Staphylococcus aureus
MTAN
-
 Staphylococcus aureus